Anti-Salivary alpha amylase antibody [EPR19605]

7 product images

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  Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Human salivary alpha-amylase contains aa311-511 with a GST-tag. Human pancreatic alpha-amylase and alpha-carcinoid alpha-amylase fragment recombinant proteins contain aa311-511 with a His-Tag^®. These three recombinant proteins were made in-house.

  All lanes: Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/10000 dilution

  Lane 1: Human salivary alpha-amylase fragment recombinant protein at 0.01 µg

  Lane 2: Human pancreatic alpha-amylase fragment recombinant protein at 0.01 µg

  Lane 3: Human alpha-carcinoid alpha-amylase fragment recombinant protein at 0.01 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 58 kDa

  Observed band size: 22 kDa, 50 kDa

  Exposure time: 15s

• 

  Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  The expression profile observed is consistent with what has been described in the literature (PMID: 3261213).

  Lanes 1 - 3: Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/20000 dilution

  Lanes 4 - 6: Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/1000 dilution

  Lane 1: Human parotid gland lysate at 10 µg

  Lane 2: Human fetal brain lysate at 10 µg

  Lane 3: Human fetal liver lysate at 10 µg

  Lane 4: Human fetal heart lysate at 10 µg

  Lane 5: Human fetal kidney lysate at 10 µg

  Lane 6: Human fetal spleen lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

  Predicted band size: 36 kDa, 58 kDa

  Observed band size: 57 kDa

  Exposure time: 3min

• 

  Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/5000 dilution

  All lanes: Human pancreas lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

  Predicted band size: 58 kDa

  Observed band size: 57 kDa

  Exposure time: 10s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

  Immunohistochemical analysis of paraffin-embedded human parotid gland tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on human parotid gland is observed. Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

  Immunohistochemical analysis of paraffin-embedded human colon tissue with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
  

  Negative control: No staining on human colon.

  Counter stained with Hematoxylin.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

  Salivary alpha amylase western blot using anti-Salivary alpha amylase antibody [EPR19605] ab201450. Publication image and figure legend from Odanaka, H., Obama, T., et al., 2020, Biol Res, PubMed 31941552.

  
  

  ab201450 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab201450 please see the product overview.

  Detection of pellicle proteins by western blot. Samples (5 μg/lane) from pellicle, GCF, parotid gland, and mixed gland, were subjected to SDS-PAGE. Three pellicle proteins, cystatin S (a), α-amylase (b), and serotransferrin (c), were examined by western blot