Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (AB251340)

This data was developed using ab201450, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (AB251340)

This data was developed using ab201450, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human parotid gland tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human parotid gland is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (AB251340)

This data was developed using ab201450, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : No staining on human colon.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (AB251340)

This data was developed using ab201450, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (<a href='/en-us/products/primary-antibodies/salivary-alpha-amylase-antibody-epr19605-ab201450'>ab201450</a>) at 1/5000 dilution

All lanes:

Human pancreas lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 57 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (AB251340)

This data was developed using ab201450, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 3261213).

Lanes 1 - 3:

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (<a href='/en-us/products/primary-antibodies/salivary-alpha-amylase-antibody-epr19605-ab201450'>ab201450</a>) at 1/20000 dilution

Lanes 4 - 6:

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (<a href='/en-us/products/primary-antibodies/salivary-alpha-amylase-antibody-epr19605-ab201450'>ab201450</a>) at 1/1000 dilution

Lane 1:

Human parotid gland lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Lane 6:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 36 kDa,58 kDa

Observed band size: 57 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (AB251340)

This data was developed using ab201450, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human salivary alpha-amylase contains aa311-511 with a GST-tag. Human pancreatic alpha-amylase and alpha-carcinoid alpha-amylase fragment recombinant proteins contain aa311-511 with a His-Tag^®. These three recombinant proteins were made in-house.

All lanes:

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (<a href='/en-us/products/primary-antibodies/salivary-alpha-amylase-antibody-epr19605-ab201450'>ab201450</a>) at 1/10000 dilution

Lane 1:

Human salivary alpha-amylase fragment recombinant protein at 0.01 µg

Lane 2:

Human pancreatic alpha-amylase fragment recombinant protein at 0.01 µg

Lane 3:

Human alpha-carcinoid alpha-amylase fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 22 kDa,50 kDa

false

Exposure time: 15s