Anti-SALL1 antibody

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SALL1 antibody (AB31526)

ab31526 staining SALL1 in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31526 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• WB

Ap

Western blot - Anti-SALL1 antibody (AB31526)

Although the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2) : 1037-45 PMID : 21062744

All lanes:

Western blot - Anti-SALL1 antibody (ab31526) at 1 µg/mL

All lanes:

E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 140 kDa

Observed band size: 130 kDa,200 kDa

false

Exposure time: 2min

• WB

Lab

Western blot - Anti-SALL1 antibody (AB31526)

Blocking buffer : 2% BSA block

Gel type : TA

All lanes:

Western blot - Anti-SALL1 antibody (ab31526) at 1 µg/mL

All lanes:

Mouse embryonic stem cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 140 kDa

Observed band size: 200 kDa

false

Exposure time: 8min