Rabbit Recombinant Multiclonal SALL4 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Transfected cell line samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunocytochemistry-immunofluorescence-img423433.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423411.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423410.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423409.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423408.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (AB315177)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Expected | Expected | Tested | Not recommended |
| Rat | Expected | Expected | Tested | Not recommended |
| Transfected cell line | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.
Alternative names
ZNF797, SALL4, Sal-like protein 4, Zinc finger protein 797, Zinc finger protein SALL4
Recommended products
Rabbit Recombinant Multiclonal SALL4 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Transfected cell line samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1097
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315177 is the carrier-free version of Anti-Sall4 antibody [RM1097] ab315176.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunocytochemistry-immunofluorescence-img423433.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
Sall4 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Sall4 antibody
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/100 (5.07 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing cytoplasmic staining in NCCIT cell line. Negative control: 293T. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1ug/ ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/mL) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423411.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human pancreas. The section was incubated with Anti-Sall4 antibody [RM1097] ab315176 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423410.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a SALL4 expression vector containing a His tag. (B) HEK-293T transfected with an empty vector. tissue labeling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a SALL4 expression vector containing a His tag. No staining on (B) HEK-293T transfected with an empty vector. The section was incubated with Anti-Sall4 antibody [RM1097] ab315176 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423409.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat testis. The section was incubated with Anti-Sall4 antibody [RM1097] ab315176 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423408.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/500 (1.014 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse testis. The section was incubated with Anti-Sall4 antibody [RM1097] ab315176 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423407.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human testis. The section was incubated with Anti-Sall4 antibody [RM1097] ab315176 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423406.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human testis seminoma tissue labeling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human testis seminoma. The section was incubated with Anti-Sall4 antibody [RM1097] ab315176 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Flow Cytometry (Intracellular) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--flow-cytometry-intracellular-img423446.jpg "Flow Cytometry (Intracellular) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
Flow Cytometry (Intracellular) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)
This data was developed using Anti-Sall4 antibody [RM1097] ab315176, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell, Left) / NCCIT (human pluripotent embryonic carcinoma epithelial cell, Right) cells labelling Sall4 with Anti-Sall4 antibody [RM1097] ab315176 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: 293T.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423407.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423406.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
![Flow Cytometry (Intracellular) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177), expandable thumbnail](https://content.abcam.com/products/images/sall4-antibody-rm1097-bsa-and-azide-free-ab315177--flow-cytometry-intracellular-img423446.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Sall4 antibody [RM1097] - BSA and Azide free (ab315177)")
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