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AB243104

Anti-Salmonella LPS antibody [se-01]

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(1 Publication)

Mouse Monoclonal Salmonella LPS antibody. Suitable for WB, ELISA and reacts with Salmonella enteritidis, Purified native protein - Salmonella enteritidis, Salmonella typhimurium samples. Cited in 1 publication.
4 Images
ELISA - Anti-Salmonella LPS antibody [se-01] (AB243104)
  • ELISA

Supplier Data

ELISA - Anti-Salmonella LPS antibody [se-01] (AB243104)

Titration of ab243104 by indirect ELISA using crude extract of S. Enteritidis.

The wells of the plate were coated with crude extract of S. Enteritidis (100 μL, 1 μg/mL). After blocking with 5% skimmed milk, 100 μl of antibody at the indicated dilution was added to the each well. HRP-conjugate goat anti-mouse IgG (100 μL, X2000 dilution) was added. Color was developed with orthophenylenediamine as substrate. Optical densities (OD) measured at 490nm.

ELISA - Anti-Salmonella LPS antibody [se-01] (AB243104)
  • ELISA

Supplier Data

ELISA - Anti-Salmonella LPS antibody [se-01] (AB243104)

Titration of LPS in the extract of S. Enteritidis cells by indirect ELISA.

The plate was coated with indicated amounts of the extract of S. Enteriditis cells. ab243104 was used at a 1/500 dilution.

Western blot - Anti-Salmonella LPS antibody [se-01] (AB243104)
  • WB

Supplier Data

Western blot - Anti-Salmonella LPS antibody [se-01] (AB243104)

Reactivites of ab243104 with several food poisoning bacteria in Western Blot (Panel A) and Slot Blot (Panel B).

Lane 1 : S. Enteritidis

Lane 2 : V. Parahaemolyticus

Lane 3 : E. Coli

Lane 4 : E. Coli O157 : H7

Panel B : Extracts of each strain of food poisoning bacteria were coated onto 5 areas of a nitrocellulose membrane. The membrane was soaked and reacted with ab243104.

SE : S. Enteritidis, EC : E. Coli, CJ : C, Jejuni, VP : V, Parahaemolyticus.

All lanes:

Western blot - Anti-Salmonella LPS antibody [se-01] (ab243104)

false

Western blot - Anti-Salmonella LPS antibody [se-01] (AB243104)
  • WB

Supplier Data

Western blot - Anti-Salmonella LPS antibody [se-01] (AB243104)

Blocked with 5 % skimmed milk.

They showed similar band patterns characteristic of LPS, O-chain repeating unit.

All lanes:

Western blot - Anti-Salmonella LPS antibody [se-01] (ab243104) at 1/250 dilution

Lane 1:

Crude extract of S. enteritidis cells at 1 µg

Lane 2:

Purified LPS of S. enteritidis cells at 1 µg

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

se-01

Isotype

IgG1

Carrier free

No

Reacts with

Salmonella typhimurium, Salmonella enteritidis

Applications

WB, ELISA

applications

Specificity

Reacts with LPS of Salmonella enteritidis and Salmonella typhimurium. Does not react with other gram-negative food-poisoning bacteria like E. coli ,V. parahaemolyticus and Campylobacter species.

This antibody recognizes common antigenic determinants that are found in the structural components of Salmonella LPS.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Purified native protein - Salmonella enteritidis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Salmonella enteritidis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>(assay dependent)</p>" }, "Salmonella typhimurium": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>(assay dependent)</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Salmonella LPS often referred to as lipopolysaccharide is an important component of the outer membrane of Salmonella bacteria. It consists of three main parts: lipid A a core oligosaccharide and an O-antigen polysaccharide. The lipid A portion is known for its endotoxic properties and typically has a mass around 2 kDa. Salmonella LPS is expressed on the surface of Salmonella spp. facilitating the interaction with host cells during infection. Its recognition is pivotal for the innate immune response and is commonly studied in the context of Western blot analysis with anti-Salmonella antibodies.
Biological function summary

Salmonella LPS plays a critical role in bacterial virulence and survival. It is part of the outer membrane layer contributing to the structural integrity of the bacterium and protecting against hostile environmental conditions. Additionally LPS is important in the formation of biofilms enabling the bacteria to adhere to surfaces and resist antimicrobial agents. The molecule also forms complexes with proteins on the host's immune cells triggering strong immune reactions. The interactions often lead to inflammatory responses via cytokine release making it an important player in immune modulation.

Pathways

The functions of Salmonella LPS are notably involved in the toll-like receptor (TLR) signaling pathway and the NF-kappa B pathway. In the TLR pathway LPS binds to TLR4 on immune cells activating downstream signaling that leads to the production of pro-inflammatory cytokines. The NF-kappa B pathway gets activated as a consequence further amplifying the immune response. Proteins like MyD88 and TRIF are closely related to Salmonella LPS in these pathways as they serve as intermediaries in signal transduction.

Salmonella LPS is linked to conditions like septic shock and gastroenteritis. The endotoxin property of the lipid A component can lead to severe inflammatory responses which might cause septic shock when the bacterial infection becomes systemic. Salmonella LPS also plays a role in gastroenteritis a result of inflammation of the stomach and intestines primarily caused by infection. It interacts with immune receptors like TLR4 and CD14 which modulate the inflammatory response that ultimately contributes to the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Salmonella LPS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1367432 PubMed38994364

2024

The mammosphere-derived epithelial cell secretome modulates neutrophil functions in the bovine model.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca M Harman,Anja Sipka,Kelly A Oxford,Leane Oliveira,Lucas Huntimer,Daryl V Nydam,Gerlinde R Van de Walle
View all publications
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