AB121664

Anti-SAMD9 antibody

Name: Anti-SAMD9 antibody (ab121664) | Abcam
Brand: Abcam Limited
SKU: AB121664
Price: 645 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SAMD9 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAMD9 aa 1-100.

View Alternative Names

C7orf5, DRIF1, KIAA2004, OEF1, SAMD9, Sterile alpha motif domain-containing protein 9, SAM domain-containing protein 9

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling SAMD9 with ab121664 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling SAMD9 with ab121664 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SAMD9 with ab121664 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling SAMD9 with ab121664 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SAMD9 antibody (AB121664)

Loading control : Anti-PPIB

All lanes:

Western blot - Anti-SAMD9 antibody (ab121664) at 0.4 µg/mL

Lane 1:

A431 (human epidermoid carcinoma cell line) cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 184 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SAMD9 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q5K651

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAMD9 also known as sterile alpha motif domain-containing 9 is an intriguing protein with a molecular mass of approximately 180 kDa. Predominantly expressed in tissues such as bone marrow and lymph nodes it plays a role in cellular proliferation and response to stress stimuli. The protein is part of the larger family of SAMD proteins which contain sterile alpha motifs influencing protein-protein interactions and it engages in regulation steps at a molecular level.

Biological function summary

SAMD9 takes part in controlling cellular proliferation and inhibiting abnormal growth mostly acting as a growth suppressor. Its functions link to maintaining homeostasis within cells and aiding in preventing uncontrolled cell expansion. SAMD9 functions independently of any known stable protein complex performing its regulatory role in diverse cellular contexts. The protein’s involvement spans multiple cell types reflecting its broad impact on normal human physiology.

Pathways

SAMD9 interacts with critical signaling modules such as the mTOR pathway and the interferon signaling pathway. It modulates cellular responses related to growth control and stress adaptation. Interactions with other proteins like TSC1 and TSC2 within the mTOR pathway highlight SAMD9's engagement in regulating cellular responses to environmental changes and nutrient availability.

SAMD9 is associated with severe conditions like myelodysplastic syndromes and MIRAGE syndrome. These conditions link to mutations affecting the protein's regulatory capacities leading to disrupted cellular processes and disease progression. The protein shares connections with SAMD9L another related protein indicating a potential overlap in disease mechanisms and impacts. Understanding the behavior of SAMD9 in these contexts is important for developing therapeutic strategies and improving disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Double-stranded nucleic acid binding that acts as an antiviral factor by playing an essential role in the formation of cytoplasmic antiviral granules (PubMed : 25428864, PubMed : 28157624). May play a role in the inflammatory response to tissue injury and the control of extra-osseous calcification, acting as a downstream target of TNF-alpha signaling. Involved in the regulation of EGR1, in coordination with RGL2. May be involved in endosome fusion.

See full target information SAMD9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2203573 PubMed36757050

2023

SAMD9 Promotes Postoperative Recurrence of Esophageal Squamous Cell Carcinoma by Stimulating MYH9-Mediated GSK3β/β-Catenin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Hao Luo,Fu-Qiang Dai,Ren-Tao Wang,Xiao-Qing Fan,Yuan-Yuan Luo,Meng-Sheng Deng,Yulun Wang,Tan Long,Wei Guo,Bo Xu,Cheng-Xiong Xu,Hua Jin

Nature communications 10:5805 PubMed31862882

2019

RNF208, an estrogen-inducible E3 ligase, targets soluble Vimentin to suppress metastasis in triple-negative breast cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoungwha Pang,Jinah Park,Sung Gwe Ahn,Jihee Lee,Yuna Park,Akira Ooshima,Seiya Mizuno,Satoshi Yamashita,Kyung-Soon Park,So-Young Lee,Joon Jeong,Toshikazu Ushijima,Kyung-Min Yang,Seong-Jin Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAMD9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (AB121664)

Western blot - Anti-SAMD9 antibody (AB121664)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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