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Rabbit Recombinant Monoclonal SAMD9 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

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Images

Western blot - Anti-SAMD9 antibody [EPR13603] (AB180575), expandable thumbnail
  • Western blot - Anti-SAMD9 antibody [EPR13603] (AB180575), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] (AB180575), expandable thumbnail
  • Western blot - Anti-SAMD9 antibody [EPR13603] (AB180575), expandable thumbnail
  • Western blot - Anti-SAMD9 antibody [EPR13603] (AB180575), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30 - 1/50
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Double-stranded nucleic acid binding that acts as an antiviral factor by playing an essential role in the formation of cytoplasmic antiviral granules (PubMed:25428864, PubMed:28157624). May play a role in the inflammatory response to tissue injury and the control of extra-osseous calcification, acting as a downstream target of TNF-alpha signaling. Involved in the regulation of EGR1, in coordination with RGL2. May be involved in endosome fusion.

Alternative names

C7orf5, DRIF1, KIAA2004, OEF1, SAMD9, Sterile alpha motif domain-containing protein 9, SAM domain-containing protein 9

Recommended products

Rabbit Recombinant Monoclonal SAMD9 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13603
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SAMD9 also known as sterile alpha motif domain-containing 9 is an intriguing protein with a molecular mass of approximately 180 kDa. Predominantly expressed in tissues such as bone marrow and lymph nodes it plays a role in cellular proliferation and response to stress stimuli. The protein is part of the larger family of SAMD proteins which contain sterile alpha motifs influencing protein-protein interactions and it engages in regulation steps at a molecular level.

Biological function summary

SAMD9 takes part in controlling cellular proliferation and inhibiting abnormal growth mostly acting as a growth suppressor. Its functions link to maintaining homeostasis within cells and aiding in preventing uncontrolled cell expansion. SAMD9 functions independently of any known stable protein complex performing its regulatory role in diverse cellular contexts. The protein’s involvement spans multiple cell types reflecting its broad impact on normal human physiology.

Pathways

SAMD9 interacts with critical signaling modules such as the mTOR pathway and the interferon signaling pathway. It modulates cellular responses related to growth control and stress adaptation. Interactions with other proteins like TSC1 and TSC2 within the mTOR pathway highlight SAMD9's engagement in regulating cellular responses to environmental changes and nutrient availability.

Associated diseases and disorders

SAMD9 is associated with severe conditions like myelodysplastic syndromes and MIRAGE syndrome. These conditions link to mutations affecting the protein's regulatory capacities leading to disrupted cellular processes and disease progression. The protein shares connections with SAMD9L another related protein indicating a potential overlap in disease mechanisms and impacts. Understanding the behavior of SAMD9 in these contexts is important for developing therapeutic strategies and improving disease management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

    Lanes 1-3: Merged signal (red and green). Green - ab180575 observed at 184 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab180575 Anti-SAMD9 antibody [EPR13603] was shown to specifically react with SAMD9 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human SAMD9 knockout A549 cell line ab267039 (knockout cell lysate Human SAMD9 knockout A549 cell lysate ab257657) was used. Wild-type and SAMD9 knockout samples were subjected to SDS-PAGE. ab180575 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: SAMD9 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human SAMD9 knockout A549 cell line (Human SAMD9 knockout A549 cell line ab267039)

    Lane 3: A431 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 184 kDa

    Observed band size: 184 kDa

  • Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

    Lanes 1-4: Merged signal (red and green). Green - ab180575 observed at 184 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab180575 Anti-SAMD9 antibody [EPR13603] was shown to specifically react with SAMD9 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human SAMD9 knockout A549 cell line ab267038 (knockout cell lysate Human SAMD9 knockout A549 cell lysate ab257656) was used. Wild-type and SAMD9 knockout samples were subjected to SDS-PAGE. ab180575 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: SAMD9 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human SAMD9 knockout A549 cell line (Human SAMD9 knockout A549 cell line ab267038)

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: Human skeletal muscle cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 184 kDa

    Observed band size: 184 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] (ab180575)

    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

    All lanes: Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution

    All lanes: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 184 kDa

    Observed band size: 184 kDa

  • Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

    All lanes: Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/10000 dilution

    Lane 1: A431 cell lysate at 20 µg with NFDM/TBST

    Lane 2: MCF-7 cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 184 kDa

  • Flow Cytometry (Intracellular) - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SAMD9 antibody [EPR13603] (ab180575)

    Intracellular Flow Cytometry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] (ab180575)

    Immunofluorescence analysis of 4% paraformaldehyde-A431 cells labeling SAMD9 with ab180575 (unpurified) (green) at 1/100 dilution. Dapi counterstain (blue). Goat anti rabbit IgG (Dylight 488) at a dilution of 1/200 was used as a secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-SAMD9 antibody [EPR13603] (ab180575), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SAMD9 antibody [EPR13603] (ab180575)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedA431 cells labeling SAMD9 with ab180575 (unpurified) at 1/50 dilution, or Rabbit monoclonal IgG Isotype control.

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Product protocols

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