AB128107

Anti-SAMHD1 antibody [OTI1A1]

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(11 Publications)

Mouse Monoclonal SAMHD1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Dog, African green monkey, Rat, Mouse, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SAMHD1.

View Alternative Names

MOP5, SAMHD1, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, Dendritic cell-derived IFNG-induced protein, Monocyte protein 5, SAM domain and HD domain-containing protein 1, DCIP, MOP-5, hSAMHD1

17 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human thyroid carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human pancreas tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY SAMHD1 overexpress plasmid (Red) or empty vector control plasmid (Blue) stained for SAMHD1 using ab128107 at 1/100 dilution.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SAMHD1 using ab128107 at 1/100 dilution (Red) compared to a nonspecific negative control (Blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human prostate carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human kidney carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human tonsil tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for SAMHD1 using ab128107 at 1/100 dilution (Red) compared to a nonspecific negative control (Blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human bladder carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human kidney tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human liver tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Paraffin-embedded human ovary adenocarcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

Lab

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Western blot : Anti-SAMHD1 antibody [OTI1A1] (ab128107) staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab128107 was shown to bind specifically to SAMHD1. A band was observed at 73/75 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in SAMHD1 knockout cell line. To generate this image, wild-type and SAMHD1 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/500 dilution

Lane 1:

HEK-293 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

SAMHD1 knockout HAP1 cell lysate at 20 µg

Lane 5:

Human Spleen cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Predicted band size: 72 kDa

false

Lab

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : SAMHD1 knockout HAP1 cell lysate (20 μg)
Lane 3 : K562 cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab128107 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab128107 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab128107 and ab181602 (loading control to GAPDH) were diluted 1/500 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Predicted band size: 72 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SAMHD1 cDNA stained forSAMHD1 using ab128107 at 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

All lanes:

Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

All lanes:

Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/200 dilution

All lanes:

Mouse spleen extract at 10 µg

Predicted band size: 72 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1A1

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

ICC/IF, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SAMHD1.

Q9Y3Z3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

The clone number has been updated from 1A1 to OTI1A1, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAMHD1 also known as Sterile Alpha Motif and HD-Domain Containing Protein 1 functions mechanically as a dGTP-dependent deoxynucleotide triphosphohydrolase. It has a molecular mass of approximately 72 kDa. This protein is expressed in various cell types most notably in immune cells such as macrophages and dendritic cells. SAMHD1 operates by hydrolyzing deoxynucleoside triphosphates (dNTPs) into deoxynucleosides and inorganic triphosphates controlling the intracellular pool of dNTPs.

Biological function summary

SAMHD1 serves as a modulator of cellular proliferation and DNA repair processes. It forms part of a larger complex that regulates DNA replication through its influence on dNTP levels. By modulating these levels the protein prevents excess dNTP buildup which can result in inefficient replication and genomic instability. Moreover SAMHD1 contributes to the innate immune response by restricting viral replication particularly that of retroviruses like HIV-1 in non-dividing cells.

Pathways

SAMHD1 participates in nucleotide metabolism and DNA damage response pathways. For effective dNTP pool regulation it interacts with proteins involved in dNTP synthesis and degradation such as RRM2B. Additionally SAMHD1 is relevant in pathways connected to the restriction of viral infections working alongside proteins like TREX1 that degrade excess DNA and sustain genomic integrity during infection.

SAMHD1 mutations associate with autoimmune disorders and certain types of cancers. Aicardi-Goutières syndrome a rare genetic inflammatory disorder shows links to defective SAMHD1 activity where impaired DNA damage response leads to chronic inflammation. Furthermore altered SAMHD1 expression relates to chronic lymphocytic leukemia where it influences both the proliferation and survival of malignant cells. Interaction with proteins such as DNA-PK is significant in linking SAMHD1 to these conditions demonstrating its role in maintaining immune system balance and genomic stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks (PubMed : 19525956, PubMed : 21613998, PubMed : 21720370, PubMed : 22056990, PubMed : 23601106, PubMed : 23602554, PubMed : 24336198, PubMed : 26294762, PubMed : 26431200, PubMed : 28229507, PubMed : 28834754, PubMed : 29670289). Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1 : dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells (PubMed : 19525956, PubMed : 21613998, PubMed : 21720370, PubMed : 22056990, PubMed : 23364794, PubMed : 23601106, PubMed : 23602554, PubMed : 24336198, PubMed : 25038827, PubMed : 26101257, PubMed : 26294762, PubMed : 26431200, PubMed : 28229507). Likewise, suppresses LINE-1 retrotransposon activity (PubMed : 24035396, PubMed : 24217394, PubMed : 29610582). Not able to restrict infection by HIV-2 virus; because restriction activity is counteracted by HIV-2 viral protein Vpx (PubMed : 21613998, PubMed : 21720370). In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools (PubMed : 23858451). Phosphorylation at Thr-592 acts as a switch to control dNTPase-dependent and -independent functions : it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks (PubMed : 23601106, PubMed : 23602554, PubMed : 29610582, PubMed : 29670289). Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks : acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication (PubMed : 29670289). Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation (PubMed : 27477283, PubMed : 29670289). Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity (PubMed : 29670289). Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (By similarity).

See full target information SAMHD1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 20:810-817 PubMed37213666

2023

Recombinant Klotho attenuates IFNγ receptor signaling and SAMHD1 expression through blocking NF-κB translocation in glomerular mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kuen-Daw Tsai,Yi-Chao Lee,Bo-Yu Chen,Li-Syuan Wu,Shan-Yuan Liang,Ming-Yuan Liu,Yu-Wen Hung,Hui-Ling Hsu,Pei-Qi Chen,Jia-Ching Shieh,Yi-Ju Lee,Ting-Hui Lin

Oncogene 42:1843-1856 PubMed37081042

2023

De-ubiquitination of SAMHD1 by USP7 promotes DNA damage repair to overcome oncogenic stress and affect chemotherapy sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Liu,Tingting Zhou,Xiang Dong,Qiqiang Guo,Lixia Zheng,Xiaoxun Wang,Naijin Zhang,Danni Li,Ling Ren,Fei Yi,Ying Zhang,Ziwei Li,Xiwen Wang,Chengsi Deng,Chunlu Li,Hongde Xu,Yi Guan,Xiaoman Li,Yang Yu,Wendong Guo,Zhuo Wang,Bo Jiang,Xuan Wu,Ning Bai,Yanling Feng,Mengtao Ma,Qingquan Kong,Jiayi Wei,Zhenshuang Wang,Hao Li,Songming Lu,Liangzi Cao,Yutong Xiao,Xiaoyu Song,Zhenning Wang,Chengzhong Xing,Liu Cao

Experimental & molecular medicine 55:779-793 PubMed37009792

2023

SAMHD1-induced endosomal FAK signaling promotes human renal clear cell carcinoma metastasis by activating Rac1-mediated lamellipodia protrusion.

Applications

Unspecified application

Species

Unspecified reactive species

Sunho An,Tam Thuy Lu Vo,Taekwon Son,Hoon Choi,Jinyoung Kim,Juyeon Lee,Byung Hoon Kim,Misun Choe,Eunyoung Ha,Young-Joon Surh,Kyu-Won Kim,Ji Hae Seo

Frontiers in endocrinology 13:1013018 PubMed36714584

2023

Growth differentiation factor 10 induces angiogenesis to promote wound healing in rats with diabetic foot ulcers by activating TGF-β1/Smad3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Zhao,Jinmei Xu,Xu Han,Zheqi Zhang,Jiahui Qu,Zhifeng Cheng

Oncology letters 24:303 PubMed35949607

2022

Expression of SAMHD1 and its mutation on prognosis of colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Zhang,Ping Li,Ping Sun

Frontiers in oncology 11:763151 PubMed34976810

2021

Mutations and Expression in Mantle Cell Lymphoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Wenqin Yue,Gusheng Tang,Mingyu Ye,Jiechen Yu,Bin Liu,Lijuan Jiao,Xuefei Liu,Shuyi Yin,Jie Chen,Lei Gao,Jianmin Yang,Miaoxia He

EMBO molecular medicine 12:e10419 PubMed31950591

2020

Ribonucleotide reductase inhibitors suppress SAMHD1 ara-CTPase activity enhancing cytarabine efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Sean G Rudd,Nikolaos Tsesmetzis,Kumar Sanjiv,Cynthia Bj Paulin,Lakshmi Sandhow,Juliane Kutzner,Ida Hed Myrberg,Sarah S Bunten,Hanna Axelsson,Si Min Zhang,Azita Rasti,Petri Mäkelä,Si'Ana A Coggins,Sijia Tao,Sharda Suman,Rui M Branca,Georgios Mermelekas,Elisée Wiita,Sun Lee,Julian Walfridsson,Raymond F Schinazi,Baek Kim,Janne Lehtiö,Georgios Z Rassidakis,Katja Pokrovskaja Tamm,Ulrika Warpman-Berglund,Mats Heyman,Dan Grandér,Sören Lehmann,Thomas Lundbäck,Hong Qian,Jan-Inge Henter,Torsten Schaller,Thomas Helleday,Nikolas Herold

Cell cycle (Georgetown, Tex.) 17:1102-1114 PubMed30039733

2018

The dNTP triphosphohydrolase activity of SAMHD1 persists during S-phase when the enzyme is phosphorylated at T592.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Tramentozzi,Paola Ferraro,Manzar Hossain,Bruce Stillman,Vera Bianchi,Giovanna Pontarin

Journal of virology 91: PubMed28148784

2017

Different Expression of Interferon-Stimulated Genes in Response to HIV-1 Infection in Dendritic Cells Based on Their Maturation State.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Calonge,Mercedes Bermejo,Francisco Diez-Fuertes,Isabelle Mangeot,Nuria González,Mayte Coiras,Laura Jiménez Tormo,Javier García-Perez,Nathalie Dereuddre-Bosquet,Roger Le Grand,José Alcamí

Journal of neuroinflammation 12:66 PubMed25890101

2015

Sterile alpha motif and histidine/aspartic acid domain-containing protein 1 (SAMHD1)-facilitated HIV restriction in astrocytes is regulated by miRNA-181a.

Applications

Flow Cyt

Species

Unspecified reactive species

Sudheesh Pilakka-Kanthikeel,Andrea Raymond,Venkata Subba Rao Atluri,Vidya Sagar,Shailendra K Saxena,Patricia Diaz,Semithe Chevelon,Michael Concepcion,Madhavan Nair

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SAMHD1 antibody [OTI1A1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Immunocytochemistry/ Immunofluorescence - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Western blot - Anti-SAMHD1 antibody [OTI1A1] (AB128107)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise