AB167430

Anti-SAMM50/SAM50 antibody [EPR8717]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal SAMM50/SAM50 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

SAM50, CGI-51, TRG3, SAMM50, Sorting and assembly machinery component 50 homolog, Transformation-related gene 3 protein, TRG-3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human stomach tissue labeling SAMM50/SAM50 with ab167430 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling SAMM50/SAM50 with ab167430 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

All lanes:

Western blot - Anti-SAMM50/SAM50 antibody [EPR8717] (ab167430) at 1/50000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

false

Carrier free

Anti-SAMM50/SAM50 antibody [EPR8717] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8717

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAMM50 also known as SAM50 is an important component in mitochondrial function. This protein is part of the sorting and assembly machinery (SAM) complex and is necessary for the insertion of β-barrel proteins into the mitochondrial outer membrane. SAMM50 has a molecular mass of approximately 50 kDa. It is predominantly expressed in tissues with high metabolic activity such as the liver and skeletal muscle where mitochondrial function is essential.

Biological function summary

SAMM50 functions as an integral part of the SAM complex which includes other essential subunits like SAM35 and SAM37. This complex facilitates the import and assembly of proteins into the mitochondrial outer membrane important for maintaining mitochondrial structure and function. By ensuring correct protein placement in the outer membrane SAMM50 supports mitochondrial membrane integrity and energy production processes.

Pathways

SAMM50 is involved in essential cellular pathways like mitochondrial protein import and metabolism. SAMM50 interacts with proteins such as TOM40 a component of the translocase of the outer membrane complex which assists in protein transport into mitochondrial compartments. These pathways contribute to the overall cellular energy balance and metabolic regulation reflecting the importance of SAMM50 in maintaining cellular homeostasis.

Alterations in SAMM50 expression or function relate to conditions like mitochondrial myopathy and liver disease. These disorders are linked to defective mitochondrial function due to improper protein import and assembly. SAMM50's interaction with other mitochondrial proteins such as TIM23 complex components influences these conditions by impacting the efficiency and functionality of mitochondrial protein import and assembly processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes (PubMed : 22252321, PubMed : 25781180). Required for the assembly of TOMM40 into the TOM complex (PubMed : 15644312).

See full target information SAMM50

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:115038 PubMed39630581

2024

OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase.

Applications

Unspecified application

Species

Unspecified reactive species

Praveenraj Elancheliyan,Klaudia K Maruszczak,Remigiusz Adam Serwa,Till Stephan,Ahmet Sadik Gulgec,Mayra A Borrero-Landazabal,Sonia Ngati,Aleksandra Gosk,Stefan Jakobs,Michal Wasilewski,Agnieszka Chacinska

mSphere 2: PubMed28567444

2017

MAF1b Binds the Host Cell MIB Complex To Mediate Mitochondrial Association.

Applications

Unspecified application

Species

Unspecified reactive species

Felice D Kelly,Brian M Wei,Alicja M Cygan,Michelle L Parker,Martin J Boulanger,John C Boothroyd

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAMM50/SAM50 antibody [EPR8717]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

Flow Cytometry (Intracellular) - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

Western blot - Anti-SAMM50/SAM50 antibody [EPR8717] (AB167430)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise