Rabbit Recombinant Monoclonal SAMM50/SAM50 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--western-blot-img113976.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108503.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117189.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626)")
![OI-RD Scanning - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--oi-rd-scanning-img85202.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (AB248626)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform antigen retrieval before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes (PubMed:22252321, PubMed:25781180). Required for the assembly of TOMM40 into the TOM complex (PubMed:15644312).
Alternative names
SAM50, CGI-51, TRG3, SAMM50, Sorting and assembly machinery component 50 homolog, Transformation-related gene 3 protein, TRG-3
Recommended products
Rabbit Recombinant Monoclonal SAMM50/SAM50 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8718
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.3 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248626 is the carrier-free version of Anti-SAMM50/SAM50 antibody [EPR8718] ab133709.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SAMM50 also known as SAM50 is an important component in mitochondrial function. This protein is part of the sorting and assembly machinery (SAM) complex and is necessary for the insertion of β-barrel proteins into the mitochondrial outer membrane. SAMM50 has a molecular mass of approximately 50 kDa. It is predominantly expressed in tissues with high metabolic activity such as the liver and skeletal muscle where mitochondrial function is essential.
- Biological function summary
SAMM50 functions as an integral part of the SAM complex which includes other essential subunits like SAM35 and SAM37. This complex facilitates the import and assembly of proteins into the mitochondrial outer membrane important for maintaining mitochondrial structure and function. By ensuring correct protein placement in the outer membrane SAMM50 supports mitochondrial membrane integrity and energy production processes.
- Pathways
SAMM50 is involved in essential cellular pathways like mitochondrial protein import and metabolism. SAMM50 interacts with proteins such as TOM40 a component of the translocase of the outer membrane complex which assists in protein transport into mitochondrial compartments. These pathways contribute to the overall cellular energy balance and metabolic regulation reflecting the importance of SAMM50 in maintaining cellular homeostasis.
- Associated diseases and disorders
Alterations in SAMM50 expression or function relate to conditions like mitochondrial myopathy and liver disease. These disorders are linked to defective mitochondrial function due to improper protein import and assembly. SAMM50's interaction with other mitochondrial proteins such as TIM23 complex components influences these conditions by impacting the efficiency and functionality of mitochondrial protein import and assembly processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--western-blot-img113976.jpg "Western blot - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)")
Western blot - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)
This data was developed using Anti-SAMM50/SAM50 antibody [EPR8718] ab133709, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SAMM50/SAM50 antibody [EPR8718] (Anti-SAMM50/SAM50 antibody [EPR8718] ab133709) at 1/1000 dilution
Lane 1: Human fetal heart tissue lysate at 10 µg
Lane 2: A431 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108503.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)
This data was developed using Anti-SAMM50/SAM50 antibody [EPR8718] ab133709, the same antibody clone in a different buffer formulation.Anti-SAMM50/SAM50 antibody [EPR8718] ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human kidney tissue using immunohistochemical analysis. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117189.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)
This data was developed using Anti-SAMM50/SAM50 antibody [EPR8718] ab133709, the same antibody clone in a different buffer formulation.Anti-SAMM50/SAM50 antibody [EPR8718] ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human ovarian carcinoma tissue using immunohistochemical analysis. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626), expandable thumbnail](https://content.abcam.com/products/images/samm50-sam50-antibody-epr8718-bsa-and-azide-free-ab248626--oi-rd-scanning-img85202.jpg "OI-RD Scanning - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)")
OI-RD Scanning - Anti-SAMM50/SAM50 antibody [EPR8718] - BSA and Azide free (ab248626)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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