Anti-SAP102 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAP102 antibody (AB152132)

Immunofluorescent analysis of methanol-fixed A431 cells labeling SAP102 with ab152132 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP102 antibody (AB152132)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SAP102 protein at cytoplasm using ab152132 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody used at a 1/500 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody (AB152132)

Immunohistochemical analysis of paraffin embedded mouse brain tissue stained for SAP102 protein at cytoplasm with ab152132 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SAP102 antibody (AB152132)

Immunohistochemical analysis of frozen sectioned E13.5 Rat brain tissue labeling SAP102 with ab152132 at 1/250 dilution.

beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody at a 1/500 dilution. DAPI was used for nuclear staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody (AB152132)

Immunohistochemical analysis of paraffin embedded mouse brain tissue stained for SAP102 protein at cytoplasm with ab152132 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP102 antibody (AB152132)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling SAP102 protein at cell body and synaptic vesicles using ab152132 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody used at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

• WB

Unknown

Western blot - Anti-SAP102 antibody (AB152132)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SAP102 antibody (ab152132) at 1/1000 dilution

All lanes:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 90 kDa

false

• WB

Unknown

Western blot - Anti-SAP102 antibody (AB152132)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SAP102 antibody (ab152132) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 90 kDa

false

• WB

Supplier Data

Western blot - Anti-SAP102 antibody (AB152132)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SAP102 antibody (ab152132) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysates at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Predicted band size: 90 kDa

false