AB288443

Anti-SAP102 antibody [EPR25330-36] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SAP102 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

KIAA1232, DLG3, Disks large homolog 3, Neuroendocrine-DLG, Synapse-associated protein 102, XLMR, SAP-102, SAP102

2 Images

Lab

Western blot - Anti-SAP102 antibody [EPR25330-36] - BSA and Azide free (AB288443)

This data was developed using ab288436, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : Negative control : heart(PMID : 8780649).

Bands below 100 kDa may due to degradation.

3 minutes

Exposure time :

All lanes:

Western blot - Anti-SAP102 antibody [EPR25330-36] (<a href='/en-us/products/primary-antibodies/sap102-antibody-epr25330-36-ab288436'>ab288436</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 90 kDa

Observed band size: 100 kDa

false

Lab

Western blot - Anti-SAP102 antibody [EPR25330-36] - BSA and Azide free (AB288443)

This data was developed using ab288436, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : Negative control : skeletal muscle;heart(PMID : 8780649).

3 minutes

Exposure time :

All lanes:

Western blot - Anti-SAP102 antibody [EPR25330-36] (<a href='/en-us/products/primary-antibodies/sap102-antibody-epr25330-36-ab288436'>ab288436</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysates at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysates at 20 µg

Lane 3:

Mouse heart tissue lysates at 20 µg

Lane 4:

Rat brain tissue lysates at 20 µg

Lane 5:

Rat skeletal muscle tissue lysates at 20 µg

Lane 6:

Rat heart tissue lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 90 kDa

false

Unconjugated

Anti-SAP102 antibody [EPR25330-36]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25330-36

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAP102 also known as DLG3 is a member of the membrane-associated guanylate kinase (MAGUK) protein family. This protein weighs approximately 97 kDa and is mainly expressed in the brain. SAP102 plays a significant role in signal transduction by organizing synaptic signaling complexes. It contains PDZ domains that facilitate protein-protein interactions and its expression in neuronal synapses is notable.

Biological function summary

The interaction between SAP102 and N-methyl-D-aspartate (NMDA) receptors has major implications in synapse development and plasticity. SAP102 forms part of the postsynaptic density (PSD) complex which contributes to synaptic organization and dynamics. This complex is essential for the correct functioning of synaptic signaling pathways influencing learning and memory processes.

Pathways

The regulation of synaptic plasticity and neurotransmitter release involves SAP102. In this context it plays a part in the NMDA receptor signaling pathway and the synaptic vesicle cycle. SAP102 is associated with proteins such as PSD95 and SAP97 which work together to modulate synaptic strength and communication.

Abnormalities in SAP102 function or expression have links to neurodevelopmental disorders like intellectual disability and autism spectrum disorder. Mutations in SAP102 and related proteins such as neuroligins or neurexins can disrupt synaptic communication leading to defective neural circuit formation. Understanding SAP102's role in these disorders offers insights into potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling.

See full target information DLG3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAP102 antibody [EPR25330-36] - BSA and Azide free

Western blot - Anti-SAP102 antibody [EPR25330-36] - BSA and Azide free (AB288443)

Western blot - Anti-SAP102 antibody [EPR25330-36] - BSA and Azide free (AB288443)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise