Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free

18 product images

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  Dot Blot - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.
  

  Dot blot analysis of SAP102 using Anti-SAP102 antibody [EPR28704-52] ab316858 at 1:1000 (0.498 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

  Lane 1: His-tagged mouse SAP102 fragment
  
  Lane 2: His-tagged mouse SAP97 fragment
  
  Lane 3: His-tagged mouse PSD93 fragment
  
  Lane 4: His-tagged mouse PSD95 fragment

  This antibody does not cross-react with mouse SAP97, PSD93 and PSD95.

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  All lanes: Dot Blot - Anti-SAP102 antibody [EPR28704-52] (Anti-SAP102 antibody [EPR28704-52] ab316858) at 1/1000 dilution

  Lane 1: His-tagged mouse SAP102 fragment

  Lane 2: His-tagged mouse SAP97 fragment

  Lane 3: His-tagged mouse PSD93 fragment

  Lane 4: His-tagged mouse PSD95 fragment

  Secondary

  All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Exposure time: 180s

• 

  Western blot - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-SAP102 antibody [EPR28704-52] (Anti-SAP102 antibody [EPR28704-52] ab316858) at 1/1000 dilution

  Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 100 kDa, 36 kDa

  Exposure time: 180s

• 

  Western blot - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Negative control: skeletal muscle (PMID: 8780649), heart (PMID: 8780649), liver (PMID: 8780649), spleen, testis.

  In lanes 1-9, the lysate was stored at -80℃ prior to Western Blotting. The bands beneath the target band (100 kDa) are likey to be degradation products. In lanes 10-17, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

  Lanes 1-7, 10-17 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 8-9 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-SAP102 antibody [EPR28704-52] (Anti-SAP102 antibody [EPR28704-52] ab316858) at 1/1000 dilution

  Lane 1: Mouse hippocampus tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 2: Mouse cerebellum tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 3: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 4: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 5: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 6: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 7: Rat skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 8: Human brain tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 9: Human liver tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 10: mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 11: mouse liver tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 12: mouse testis tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 13: mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 14: rat brain tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 15: rat liver tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 16: rat testis tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 17: rat spleen tissue lysate at 20 µg with 5% NFDM/TBST

  Secondary

  Lanes 1, 2, 3, 4, 5, 6, 7, 10, 11, 12, 13, 14, 15, 16 and 17: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Lanes 8 - 9: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 100 kDa, 36 kDa

  Exposure time: 8s

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/50 (9.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in rat primary neuron.
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/50 (9.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neuron.
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Negative control: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocmapus (fresh frozen) tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-SAP102 (Anti-SAP102 antibody [EPR28704-52] ab316858, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-SAP102 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Negative control: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• 

  Flow Cytometry (Intracellular) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells labelling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

• 

  Flow Cytometry (Intracellular) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

• 

  Immunohistochemistry (Frozen sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocmapus (fresh frozen) tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-SAP102 (Anti-SAP102 antibody [EPR28704-52] ab316858, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-SAP102 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining on rat liver.
  
  The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining on rat skeletal muscle.
  
  The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining on mouse liver.
  
  The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining on mouse skeletal muscle.
  
  The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat hippocampus (PMID: 8780649).
  
  The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse brain cortex tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse brain cortex.
  
  The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody [EPR28704-52] - BSA and Azide free (ab316859)

  This data was developed using Anti-SAP102 antibody [EPR28704-52] ab316858, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling SAP102 with Anti-SAP102 antibody [EPR28704-52] ab316858 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse hippocampus.
  
  The section was incubated with Anti-SAP102 antibody [EPR28704-52] ab316858 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins