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AB249889

Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SAP18 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

GIG38, SAP18, Histone deacetylase complex subunit SAP18, 18 kDa Sin3-associated polypeptide, 2HOR0202, Cell growth-inhibiting gene 38 protein, Sin3-associated polypeptide p18

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free (AB249889)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free (AB249889)

This data was developed using ab175920, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of 293T cells labeling SAP18 with ab175920 at 1/100 dilution.

Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free (AB249889)
  • IP

Supplier Data

Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free (AB249889)

This data was developed using ab175920, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling SAP18 with ab175920 at 1/10 dilution

All lanes:

Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (<a href='/en-us/products/primary-antibodies/sap18-antibody-epr12956b-ab175920'>ab175920</a>)

Predicted band size: 18 kDa,79 kDa,90 kDa

false

Western blot - Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free (AB249889)
  • WB

Supplier Data

Western blot - Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free (AB249889)

This data was developed using ab175920, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SAP18 antibody [EPR12956(B)] (<a href='/en-us/products/primary-antibodies/sap18-antibody-epr12956b-ab175920'>ab175920</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Hela cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 5:

PC-12 cell lysate at 10 µg

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12956(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249889 is the carrier-free version of ab175920.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target SAP18 also known as Sin3A-associated protein 18 kDa functions as a part of a critical molecular machinery. With an approximate mass of 18 kilodaltons SAP18 acts as a component of the Sin3A corepressor complex which has roles in the regulation of gene expression. It is expressed in a variety of tissues with significant expression detected in neuronal cells. Through its interaction with other proteins in the complex SAP18 contributes to the repression of specific genes by altering chromatin structure.
Biological function summary

Proteins such as SAP18 influence essential processes by serving as a component of the Sin3A/histone deacetylase (HDAC) complexes. These complexes facilitate transcriptional repression by removing acetyl groups from histone proteins which leads to chromatin condensation and reduced gene activity. SAP18 as part of these complexes aids in regulating genes involved in cell differentiation apoptosis and development. Its role in these processes highlights its importance in maintaining cellular functions and homeostasis.

Pathways

The role of SAP18 in gene expression regulation supports its involvement in key pathways such as cell cycle control and signal transduction. In the context of the cell cycle pathway SAP18 and its complexes work closely with other regulatory proteins like Rb and E2F to ensure proper cell cycle progression by modulating transcription of cycle-dependent genes. In signal transduction pathways SAP18 has the capacity to interact with proteins like HDAC1 influencing the downstream signaling that impacts cell fate decisions.

Researchers have linked SAP18 aberrations to conditions such as cancer and neurodegenerative diseases. In cancer dysregulation of SAP18 expression or function can lead to uncontrolled cell proliferation due to a failure in proper gene repression involving interactions with proteins like HDAC1 and Rb. In neurodegenerative diseases altered SAP18 activity can contribute to neuronal cell death with connections to proteins within the Sin3A/HDAC complex that are important in neuronal survival pathways. These associations highlight SAP18 as a potential therapeutic target in relevant pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit mRNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits the formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function.
See full target information SAP18
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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