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Rabbit Recombinant Monoclonal SAP97 antibody. Suitable for IP, IHC-Fr, IHC-P, WB and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-FrIHC-PWB
Human
Tested
Not recommended
Not recommended
Expected
Tested
Tested
Mouse
Tested
Not recommended
Not recommended
Tested
Tested
Tested
Rat
Expected
Not recommended
Not recommended
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). May also play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane (PubMed:23676497).

Alternative names

Disks large homolog 1, Synapse-associated protein 97, hDlg, SAP-97, SAP97, DLG1

Recommended products

Rabbit Recombinant Monoclonal SAP97 antibody. Suitable for IP, IHC-Fr, IHC-P, WB and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26467-123
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SAP97 also known as Synapse-associated protein 97 or DLG1 is a member of the membrane-associated guanylate kinase (MAGUK) family. It has a molecular mass of approximately 97 kDa. This protein is well-expressed in several tissues with high levels observed within the brain and cardiac muscles. SAP97 plays an essential role in organizing synaptic proteins and maintaining cell polarity by aiding in the trafficking and localization of certain ion channels and receptors across the plasma membrane.

Biological function summary

The function of SAP97 is closely linked to synaptic plasticity and signal transduction. This protein forms part of larger protein complexes that include ion channels and receptors helping regulate the transmission of signals between neurons. In the brain SAP97 facilitates the clustering of neurotransmitter receptors at synapses including AMPA receptors which are vital for excitatory synaptic transmission. Its role extends to modulating the structural organization and functional regulation of these protein complexes at synaptic junctions.

Pathways

SAP97 is involved in key signaling processes particularly the glutamatergic synapse pathway where it influences the trafficking and surface expression of glutamate receptors. It interacts with other MAGUK family members like PSD-95 to regulate the assembly of the postsynaptic density. This protein also plays a role in the cardiac signaling cascade particularly in interactions involving potassium channels impacting the cardiac action potential.

Associated diseases and disorders

SAP97 has associations with neurological and cardiac conditions. Abnormal expression or mutation in SAP97 can contribute to disorders such as schizophrenia and arrhythmias. In schizophrenia disruption in SAP97 function affects synaptic communication with related proteins like PSD-95 implicated in the disease's pathology. In cardiac arrhythmias the misregulation of SAP97 interaction with ion channels might result in altered cardiac electrical activity impacting the role of proteins such as Kv4.2 in the heart's electrophysiological stability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 130 kDa

  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse liver (PMID: 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum (PMID: 7891172, PMID: 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID: 7891172, PMID: 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAP97 with ab300481 at 1/2000 (0.234 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon (PMID: 7891172). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate 20 µg

    Lane 2: HepG2 transfected with siRNAs specifically targeti DLG1, whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 130 kDa

    Exposure time: 15s

  • Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481), expandable thumbnail

    Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The blot of lane 6 was developed using a high sensitivity ECL substrate.The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8755482).

    All lanes: Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 20 µg

    Lane 3: Mouse brain tissue lysate 20 µg

    Lane 4: Mouse liver tissue lysate 20 µg

    Lane 5: Rat brain tissue lysate 20 µg

    Lane 6: Human cerebellum tissue lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 130 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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