Anti-SAP97 antibody [EPR26467-123]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAP97 with ab300481 at 1/2000 (0.234 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon (PMID : 7891172). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

Lab

Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)

SAP97 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg with ab300481 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg (Inset) Lane 2 : ab300481 IP in SH-SY5Y whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300481 in SH-SY5Y whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5.5 seconds

All lanes:

Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/30 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate (Inset) at 10 µg

Lane 2:

ab300481 IP in SH-SY5Y whole cell lysate

Lane 3:

Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 5.5s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse liver (PMID : 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID : 7891172, PMID : 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum (PMID : 7891172, PMID : 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

• IP

Lab

Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)

SAP97 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 µg with ab300481 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate 10 µg (Inset) Lane 2 : ab300481 IP in Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300481 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5.5 seconds

All lanes:

Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/30 dilution

Lane 1:

Mouse brain tissue lysate (Inset) at 10 µg

Lane 2:

Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (ab300481)

Lane 3:

Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 5.5s

• WB

Supplier Data

Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate 20 µg

Lane 2:

HepG2 transfected with siRNAs specifically targeti DLG1, whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa

true

• WB

Supplier Data

Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The blot of lane 6 was developed using a high sensitivity ECL substrate.The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8755482).

All lanes:

Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 20 µg

Lane 3:

Mouse brain tissue lysate 20 µg

Lane 4:

Mouse liver tissue lysate 20 µg

Lane 5:

Rat brain tissue lysate 20 µg

Lane 6:

Human cerebellum tissue lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa

false