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AB248705

Anti-SAP97 antibody [EPR6821] - BSA and Azide free

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Rabbit Recombinant Monoclonal SAP97 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Disks large homolog 1, Synapse-associated protein 97, hDlg, SAP-97, SAP97, DLG1

2 Images
Western blot - Anti-SAP97 antibody [EPR6821] - BSA and Azide free (AB248705)
  • WB

Unknown

Western blot - Anti-SAP97 antibody [EPR6821] - BSA and Azide free (AB248705)

This data was developed using ab134156, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SAP97 antibody [EPR6821] (<a href='/en-us/products/primary-antibodies/sap97-antibody-epr6821-ab134156'>ab134156</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 100 kDa

false

OI-RD Scanning - Anti-SAP97 antibody [EPR6821] - BSA and Azide free (AB248705)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SAP97 antibody [EPR6821] - BSA and Azide free (AB248705)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6821

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248705 is the carrier-free version of ab134156.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAP97 also known as Synapse-associated protein 97 or DLG1 is a member of the membrane-associated guanylate kinase (MAGUK) family. It has a molecular mass of approximately 97 kDa. This protein is well-expressed in several tissues with high levels observed within the brain and cardiac muscles. SAP97 plays an essential role in organizing synaptic proteins and maintaining cell polarity by aiding in the trafficking and localization of certain ion channels and receptors across the plasma membrane.
Biological function summary

The function of SAP97 is closely linked to synaptic plasticity and signal transduction. This protein forms part of larger protein complexes that include ion channels and receptors helping regulate the transmission of signals between neurons. In the brain SAP97 facilitates the clustering of neurotransmitter receptors at synapses including AMPA receptors which are vital for excitatory synaptic transmission. Its role extends to modulating the structural organization and functional regulation of these protein complexes at synaptic junctions.

Pathways

SAP97 is involved in key signaling processes particularly the glutamatergic synapse pathway where it influences the trafficking and surface expression of glutamate receptors. It interacts with other MAGUK family members like PSD-95 to regulate the assembly of the postsynaptic density. This protein also plays a role in the cardiac signaling cascade particularly in interactions involving potassium channels impacting the cardiac action potential.

SAP97 has associations with neurological and cardiac conditions. Abnormal expression or mutation in SAP97 can contribute to disorders such as schizophrenia and arrhythmias. In schizophrenia disruption in SAP97 function affects synaptic communication with related proteins like PSD-95 implicated in the disease's pathology. In cardiac arrhythmias the misregulation of SAP97 interaction with ion channels might result in altered cardiac electrical activity impacting the role of proteins such as Kv4.2 in the heart's electrophysiological stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). May also play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane (PubMed : 23676497).
See full target information DLG1_HUMAN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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