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AB326352

Anti-SAP97 antibody [G28]

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Rabbit Recombinant Monoclonal SAP97 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples.

View Alternative Names

Dlgh1, Dlg1, Disks large homolog 1, Synapse-associated protein 97, SAP-97, SAP97, Disks large homolog 1, Synapse-associated protein 97, hDlg, SAP-97, SAP97, DLG1, Dlgh1, Dlg1, Disks large homolog 1, Embryo-dlg/synapse-associated protein 97, Synapse-associated protein 97, E-dlg/SAP97, SAP-97, SAP97

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cells (Kelly). Species : Human. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326352 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Pheochromocytoma cells (PC-12). Species : Rat. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326352 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

Western blot - Anti-SAP97 antibody [G28] (AB326352)
  • WB

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Human SAP97 protein in HeLa whole cell lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 1 min at RT in the dark. Exposed 5 seconds.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 5s

Western blot - Anti-SAP97 antibody [G28] (AB326352)
  • WB

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Rat SAP97 protein in a Rat brain membrane lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 6.54 seconds.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

Rat brain membrane lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 6.54s

Western blot - Anti-SAP97 antibody [G28] (AB326352)
  • WB

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Mouse SAP97 protein in Mouse Brain lysate. Block : 5% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 1 second.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

Mouse Brain lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

G28

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel (By similarity). During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane (By similarity).
See full target information Dlg1

Additional targets

DLG1_HUMAN,
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com