Rabbit Polyclonal SAR1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus.
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Publications
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- The EMBO journal 41:e1105962022Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSantosh Phuyal,Elena Djaerff,Anabel-Lise Le Roux,Martin J Baker,Daniela Fankhauser,Sayyed Jalil Mahdizadeh,Veronika Reiterer,Amirabbas Parizadeh,Edward Felder,Jennifer C Kahlhofer,David Teis,Marcelo G Kazanietz,Stephan Geley,Leif Eriksson,Pere Roca-Cusachs,Hesso FarhanPubMed 35938214
- Proceedings of the National Academy of Sciences of the United States of America 117:27598-276072020Stimulator of interferon genes (STING) is an essential proviral host factor for human rhinovirus species A and C.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin L McKnight,Karen V Swanson,Kathryn Austgen,Cindy Richards,Jonathan K Mitchell,David R McGivern,Ethan Fritch,John Johnson,Katja Remlinger,Michal Magid-Slav,Maryna Kapustina,Shihyun You,Stanley M LemonPubMed 33060297
- eLife 5:2016Thrombospondin expression in myofibers stabilizes muscle membranes.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NR31
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis, Pig | Dilution info - | Notes - |
Target data
Function
Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. The active GTP-bound form inserts into the endoplasmic reticulum membrane where it recruits the remainder of the coat protein complex II/COPII. The coat protein complex II assembling and polymerizing on endoplasmic reticulum membrane is responsible for both the sorting of cargos and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed:23433038, PubMed:32358066, PubMed:36369712). The GTPase activity of SAR1 by controlling the timing of COPII budding regulates the size of the formed vesicles and is important for cargo selection depending on their size (PubMed:32358066). Together with SEC16A, forms the organized scaffold defining endoplasmic reticulum exit sites (ERES), some specific domains of the endoplasmic reticulum where COPII vesicles form (PubMed:17005010). In addition to its role in vesicle trafficking, can also function as a leucine sensor regulating TORC1 signaling and more indirectly cellular metabolism, growth and survival. In absence of leucine, interacts with the GATOR2 complex via MIOS and inhibits TORC1 signaling. The binding of leucine abrogates the interaction with GATOR2 and the inhibition of the TORC1 signaling. This function is completely independent of the GTPase activity of SAR1B (PubMed:34290409).
Alternative names
SAR1, SARA, SARA1, SAR1A, Small COPII coat GTPase SAR1A, COPII-associated small GTPase, Secretion-associated Ras-related GTPase 1A
Recommended products
Rabbit Polyclonal SAR1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NR31
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab125871 is purified by antigen-affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SAR1 also known as SAR1A or SAR1B is a small GTPase belonging to the Ras superfamily. It plays an integral role in protein transport by regulating the formation of transport vesicles from the endoplasmic reticulum (ER). SAR1 has a molecular mass of about 21 kDa. It is ubiquitously expressed with significant levels observed in tissues involved in high protein synthesis activities such as liver and pancreas. Mechanically it switches between GDP-bound inactive and GTP-bound active states which is essential for its function in vesicle formation.
- Biological function summary
SAR1 is an important component in the coat protein complex II (COPII) which is vital for ER-to-Golgi transport. Within this complex SAR1 assists in the initiation of vesicle budding by recruiting other COPII components such as Sec23/Sec24 and Sec13/Sec31. This assembly is important for maintaining efficient secretory pathways and cellular homeostasis. The COPII complex with SAR1 ensures the proper sorting and packaging of proteins destined for the Golgi apparatus.
- Pathways
SAR1 plays an essential role in the secretory pathway facilitating intracellular protein trafficking. This pathway is vital for transporting proteins from the ER to the Golgi impacting processes such as cell signaling and membrane composition. SAR1 interacts with other proteins including Sec23 and Sec24 to ensure proper vesicle formation and function. These interactions highlight SAR1's role within the broader exocytic pathway signifying its importance in maintaining cellular function.
- Associated diseases and disorders
SAR1 mutations have been linked to lipid absorption disorders such as chylomicron retention disease (CMRD) and Anderson's disease. These conditions result from defective protein trafficking leading to impaired chylomicron secretion. In CMRD SAR1 dysfunction affects the related APOB protein contributing to the accumulation of fat in enterocytes and subsequent clinical symptoms. Understanding SAR1's function in these diseases may offer insights into therapeutic strategies targeting protein transport mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SAR1 antibody (ab125871)
12% SDS PAGE
All lanes: Western blot - Anti-SAR1 antibody (ab125871) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Predicted band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SAR1 antibody (ab125871)
ab125871, at 1/500, staining SAR1 in methanol-fixed A431 cells by Immunofluorescence. Lower image is co-stained with Hoechst 33342.
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