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AB125871

Anti-SAR1 antibody

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(4 Publications)

Rabbit Polyclonal SAR1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus.

View Alternative Names

SAR1, SARA, SARA1, SAR1A, Small COPII coat GTPase SAR1A, COPII-associated small GTPase, Secretion-associated Ras-related GTPase 1A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAR1 antibody (AB125871)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAR1 antibody (AB125871)

ab125871, at 1/500, staining SAR1 in methanol-fixed A431 cells by Immunofluorescence. Lower image is co-stained with Hoechst 33342.

Western blot - Anti-SAR1 antibody (AB125871)
  • WB

Unknown

Western blot - Anti-SAR1 antibody (AB125871)

12% SDS PAGE

All lanes:

Western blot - Anti-SAR1 antibody (ab125871) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SAR1A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NR31

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab125871 is purified by antigen-affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAR1 also known as SAR1A or SAR1B is a small GTPase belonging to the Ras superfamily. It plays an integral role in protein transport by regulating the formation of transport vesicles from the endoplasmic reticulum (ER). SAR1 has a molecular mass of about 21 kDa. It is ubiquitously expressed with significant levels observed in tissues involved in high protein synthesis activities such as liver and pancreas. Mechanically it switches between GDP-bound inactive and GTP-bound active states which is essential for its function in vesicle formation.
Biological function summary

SAR1 is an important component in the coat protein complex II (COPII) which is vital for ER-to-Golgi transport. Within this complex SAR1 assists in the initiation of vesicle budding by recruiting other COPII components such as Sec23/Sec24 and Sec13/Sec31. This assembly is important for maintaining efficient secretory pathways and cellular homeostasis. The COPII complex with SAR1 ensures the proper sorting and packaging of proteins destined for the Golgi apparatus.

Pathways

SAR1 plays an essential role in the secretory pathway facilitating intracellular protein trafficking. This pathway is vital for transporting proteins from the ER to the Golgi impacting processes such as cell signaling and membrane composition. SAR1 interacts with other proteins including Sec23 and Sec24 to ensure proper vesicle formation and function. These interactions highlight SAR1's role within the broader exocytic pathway signifying its importance in maintaining cellular function.

SAR1 mutations have been linked to lipid absorption disorders such as chylomicron retention disease (CMRD) and Anderson's disease. These conditions result from defective protein trafficking leading to impaired chylomicron secretion. In CMRD SAR1 dysfunction affects the related APOB protein contributing to the accumulation of fat in enterocytes and subsequent clinical symptoms. Understanding SAR1's function in these diseases may offer insights into therapeutic strategies targeting protein transport mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. The active GTP-bound form inserts into the endoplasmic reticulum membrane where it recruits the remainder of the coat protein complex II/COPII. The coat protein complex II assembling and polymerizing on endoplasmic reticulum membrane is responsible for both the sorting of cargos and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed : 23433038, PubMed : 32358066, PubMed : 36369712). The GTPase activity of SAR1 by controlling the timing of COPII budding regulates the size of the formed vesicles and is important for cargo selection depending on their size (PubMed : 32358066). Together with SEC16A, forms the organized scaffold defining endoplasmic reticulum exit sites (ERES), some specific domains of the endoplasmic reticulum where COPII vesicles form (PubMed : 17005010). In addition to its role in vesicle trafficking, can also function as a leucine sensor regulating TORC1 signaling and more indirectly cellular metabolism, growth and survival. In absence of leucine, interacts with the GATOR2 complex via MIOS and inhibits TORC1 signaling. The binding of leucine abrogates the interaction with GATOR2 and the inhibition of the TORC1 signaling. This function is completely independent of the GTPase activity of SAR1B (PubMed : 34290409).
See full target information SAR1A
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37432316

2023

Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset.

Applications

Unspecified application

Species

Unspecified reactive species

Arian Mansur,Remi Joseph,Euri S Kim,Pierre M Jean-Beltran,Namrata D Udeshi,Cadence Pearce,Hanjie Jiang,Reina Iwase,Miroslav P Milev,Hashem A Almousa,Elyshia McNamara,Jeffrey Widrick,Claudio Perez,Gianina Ravenscroft,Michael Sacher,Philip A Cole,Steven A Carr,Vandana A Gupta

The EMBO journal 41:e110596 PubMed35938214

2022

Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1.

Applications

Unspecified application

Species

Unspecified reactive species

Santosh Phuyal,Elena Djaerff,Anabel-Lise Le Roux,Martin J Baker,Daniela Fankhauser,Sayyed Jalil Mahdizadeh,Veronika Reiterer,Amirabbas Parizadeh,Edward Felder,Jennifer C Kahlhofer,David Teis,Marcelo G Kazanietz,Stephan Geley,Leif Eriksson,Pere Roca-Cusachs,Hesso Farhan

Proceedings of the National Academy of Sciences of the United States of America 117:27598-27607 PubMed33060297

2020

Stimulator of interferon genes (STING) is an essential proviral host factor for human rhinovirus species A and C.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin L McKnight,Karen V Swanson,Kathryn Austgen,Cindy Richards,Jonathan K Mitchell,David R McGivern,Ethan Fritch,John Johnson,Katja Remlinger,Michal Magid-Slav,Maryna Kapustina,Shihyun You,Stanley M Lemon

eLife 5: PubMed27669143

2016

Thrombospondin expression in myofibers stabilizes muscle membranes.

Applications

WB

Species

Mouse

Davy Vanhoutte,Tobias G Schips,Jennifer Q Kwong,Jennifer Davis,Andoria Tjondrokoesoemo,Matthew J Brody,Michelle A Sargent,Onur Kanisicak,Hong Yi,Quan Q Gao,Joseph E Rabinowitz,Talila Volk,Elizabeth M McNally,Jeffery D Molkentin
View all publications
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Product promise

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