AB124875

Anti-SARA antibody [EPR5020]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal SARA antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

MADHIP, SARA, SMADIP, ZFYVE9, Zinc finger FYVE domain-containing protein 9, Mothers against decapentaplegic homolog-interacting protein, Novel serine protease, Receptor activation anchor, Smad anchor for receptor activation, Madh-interacting protein, NSP, hSARA

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARA antibody [EPR5020] (AB124875)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARA antibody [EPR5020] (AB124875)

Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SARA antibody [EPR5020] (AB124875)

All lanes:

Western blot - Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 156 kDa

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Western blot - Anti-SARA antibody [EPR5020] (AB124875)

All lanes:

Western blot - Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

Lane 1:

Human heart tissue lysate at 10 µg

Lane 2:

A673 cell lysate at 10 µg

Lane 3:

HT-1080 cell lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 156 kDa

false

OI-RD Scanning - Anti-SARA antibody [EPR5020] (AB124875)

OI-RD Scanning

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OI-RD Scanning - Anti-SARA antibody [EPR5020] (AB124875)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-SARA antibody [EPR5020] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5020

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARA also known as Smad anchor for receptor activation plays an important mechanical role in cellular signaling. It functions by binding to phosphorylated receptor-regulated Smad proteins (R-Smads) and facilitating their movement and accumulation in the nucleus. SARA has a molecular mass of approximately 150 kDa and is prominently expressed in a variety of tissues including the brain liver and kidney indicating a widespread functional role in physiological processes.

Biological function summary

SARA engages in interactions with several signaling molecules in the cell. It acts as a scaffolding protein that forms part of a complex with the Smad proteins assisting in the regulation of transforming growth factor-beta (TGF-β) signaling. By serving as a critical link between membranes and the cytoplasm it ensures the accurate transmission of signals necessary for cellular responses. Its location and function highlight its vital role in maintaining cellular homeostasis.

Pathways

SARA plays an important role in TGF-β and bone morphogenetic protein (BMP) signaling pathways. These pathways are essential for regulating cellular processes such as proliferation differentiation and apoptosis. SARA interacts with Smad2 Smad3 and Smad7 which are key components of these pathways. Through these interactions SARA helps mediate the transduction of signals that guide complex cellular activities and responses linking external signals to gene expression inside the nucleus.

Research has linked SARA to cancer and fibrotic diseases. Its role in TGF-β signaling suggests that alterations in SARA expression or function can contribute to tumor progression and metastatic processes. Additionally in fibrotic diseases where excess tissue scarring occurs the modulation of the pathway by proteins like Smad2 and Smad3 with SARA's involvement can result in aberrant tissue responses. Understanding SARA's involvement in these conditions may offer potential therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.

See full target information ZFYVE9

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Therapeutic advances in chronic disease 10:2040622319869116 PubMed31452866

2019

Combined melatonin and poricoic acid A inhibits renal fibrosis through modulating the interaction of Smad3 and β-catenin pathway in AKI-to-CKD continuum.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Qian Chen,Gang Cao,Hui Zhao,Lin Chen,Tian Yang,Ming Wang,Nosratola D Vaziri,Yan Guo,Ying-Yong Zhao

Neurochemical research 44:1807-1817 PubMed31093905

2019

Knockdown of microRNA-17-5p Enhances the Neuroprotective Effect of Act A/Smads Signal Loop After Ischemic Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao-Qi Wang,Yue Dong,Si-Jia Li,Cheng-Liang Pan,Hong-Yu Liu,Yu-Kai Wang,Lei Xu,Jia-Hui Yang,Yun-Xia Cui,Jin-Ting He,Jing Mang,Zhong-Xin Xu

American journal of cancer research 6:1549-62 PubMed27508097

2016

MicroRNA-17/20a impedes migration and invasion via TGF-β/ITGB6 pathway in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Jing,Gang Ma,Xukun Li,Xiaowei Wu,Furong Huang,Kuangyu Liu,Zhihua Liu

Journal of cell science 129:569-79 PubMed26729221

2016

FGF2 inhibits endothelial-mesenchymal transition through microRNA-20a-mediated repression of canonical TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ana C P Correia,Jan-Renier A J Moonen,Marja G L Brinker,Guido Krenning

Journal of cell science 128:3781-95 PubMed26306492

2015

Essential role of endocytosis for interleukin-4-receptor-mediated JAK/STAT signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Kurgonaite,Hetvi Gandhi,Thomas Kurth,Sophie Pautot,Petra Schwille,Thomas Weidemann,Christian Bökel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARA antibody [EPR5020]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARA antibody [EPR5020] (AB124875)

Western blot - Anti-SARA antibody [EPR5020] (AB124875)

Western blot - Anti-SARA antibody [EPR5020] (AB124875)

OI-RD Scanning - Anti-SARA antibody [EPR5020] (AB124875)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise