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AB249755

Anti-Sarcalumenin antibody [EPR12590] - BSA and Azide free

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Rabbit Recombinant Monoclonal Sarcalumenin antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Sarcalumenin, SRL

1 Images
Western blot - Anti-Sarcalumenin antibody [EPR12590] - BSA and Azide free (AB249755)
  • WB

Supplier Data

Western blot - Anti-Sarcalumenin antibody [EPR12590] - BSA and Azide free (AB249755)

This data was developed using ab173294, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sarcalumenin antibody [EPR12590] (<a href='/en-us/products/primary-antibodies/sarcalumenin-antibody-epr12590-ab173294'>ab173294</a>) at 1/50000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Predicted band size: 101 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12590

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249755 is the carrier-free version of ab173294.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sarcalumenin is a calcium-binding protein with a molecular weight of approximately 160 kDa. It's also known as SRL. This protein is expressed mainly in the skeletal and cardiac muscle tissues where it populates the sarcoplasmic reticulum. Sarcalumenin plays a role in modulating calcium dynamics by storing and buffering calcium ions which is critical for muscle contraction and relaxation processes.
Biological function summary

This calcium-binding protein is involved in various muscle functions by being part of the sarcoplasmic reticulum membrane complex. Sarcalumenin assists in the regulation of calcium uptake and release essential for proper muscle function. It interacts with other proteins such as calsequestrin and triadin contributing to efficient calcium cycling which is necessary for rhythmic contractions of cardiac and skeletal muscles.

Pathways

Sarcalumenin plays an integral part in the calcium signaling pathway which is fundamental for muscle contraction and energy metabolism. It works closely with proteins like SERCA (Sarcoplasmic/Endoplasmic Reticulum Ca²⁺ ATPase) to facilitate the cycling of calcium ions across the sarcoplasmic reticulum. Furthermore it contributes to the excitation-contraction coupling pathway where its interaction with calsequestrin ensures synchronized muscle activity.

Altered expression or mutation of sarcalumenin has associations with cardiac-related conditions such as arrhythmias and cardiomyopathy. The protein's interaction with RyR2 (ryanodine receptor 2) highlights its role in these disorders. In skeletal muscles abnormalities in sarcalumenin function can lead to muscle fatigue and weakened contraction sometimes observed in myopathy patients. The balance and interaction with calsequestrin also suggest its involvement in maintaining muscle health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SRL
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com