Anti-Sarcomeric Alpha Actinin antibody (ab137346) is a rabbit polyclonal antibody that is used to detect Sarcomeric Alpha Actinin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 40 publications
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- NPJ Regenerative medicine 8:602023Local decorin delivery via hyaluronic acid microrods improves cardiac performance, ventricular remodeling after myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriya Mohindra,Justin X Zhong,Qizhi Fang,Darnell L Cuylear,Cindy Huynh,Huiliang Qiu,Dongwei Gao,Bhushan N Kharbikar,Xiao Huang,Matthew L Springer,Randall J Lee,Tejal A DesaiPubMed 37872196
- International journal of nanomedicine 18:1809-182120234D Thermo-Responsive Smart hiPSC-CM Cardiac Construct for Myocardial Cell Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung Yun Hann,Haitao Cui,Timothy Esworthy,Lijie Grace ZhangPubMed 37051312
- Frontiers in medicine 9:9223472022A rare case of arrhythmogenic right ventricular cardiomyopathy associated with mutation: A case report and literature review.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Wang,Yibing Fang,Dan Zhang,Yifei Li,Shuhua LuoPubMed 35924034
- eLife 11:2022Activation of transient receptor potential vanilloid 4 is involved in pressure overload-induced cardiac hypertrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zou,Miaomiao Zhang,Qiongfeng Wu,Ning Zhao,Minwei Chen,Cui Yang,Yimei Du,Bing HanPubMed 35731090
- European journal of histochemistry : EJH 66:2022MALAT1 regulates hypertrophy of cardiomyocytes by modulating the miR-181a/HMGB2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Chen,Wenfeng Li,Dandan Zhang,Youlin Fu,Wenjin Yuan,Gang Luo,Fuwei Liu,Jun LuoPubMed 35726535
- Frontiers in cardiovascular medicine 9:8491752022Alteration of N-Methyladenosine mRNA Methylation in a Human Stem Cell-Derived Cardiomyocyte Model of Tyrosine Kinase Inhibitor-Induced Cardiotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Ma,Xian Liu,Yiming Bi,Tianhu Wang,Cheng Chen,Yabin Wang,Dong Han,Feng CaoPubMed 35402566
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e21042992022hESCs-Derived Early Vascular Cell Spheroids for Cardiac Tissue Vascular Engineering and Myocardial Infarction Treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Liu,Yifan Zhang,Tianxiao Mei,Hao Cao,Yihui Hu,Wenwen Jia,Jing Wang,Ziliang Zhang,Zhan Wang,Wenjun Le,Zhongmin LiuPubMed 35092352
- Science advances 7:eabh39952021Plakophilin-2 truncating variants impair cardiac contractility by disrupting sarcomere stability and organization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKehan Zhang,Paige E Cloonan,Subramanian Sundaram,Feng Liu,Shoshana L Das,Jourdan K Ewoldt,Jennifer L Bays,Samuel Tomp,Christopher N Toepfer,Júlia D C Marsiglia,Joshua Gorham,Daniel Reichart,Jeroen Eyckmans,Jonathan G Seidman,Christine E Seidman,Christopher S ChenPubMed 34652945
- Experimental and therapeutic medicine 22:12712021TFEC contributes to cardiac hypertrophy by inhibiting AMPK/mTOR signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhao,Zhenyu Wang,Yehong Chi,Chunmei Ni,Xudan ZhengPubMed 34594408
- Advanced biology 5:e21006092021Microfluidic Co-Culture Platform to Recapitulate the Maternal-Placental-Embryonic Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia A Boos,Patrick M Misun,Giulia Brunoldi,Lea A Furer,Leonie Aengenheister,Mario Modena,Nassim Rousset,Tina Buerki-Thurnherr,Andreas HierlemannPubMed 34145989
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACTN2. The exact immunogen used to generate this antibody is proprietary information. Database link P35609
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Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Expected | Expected | Tested |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Use ice-cold Methanol as fixation. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Species Rat | Dilution info 1/100 - 1/1000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Species Human | Dilution info 1/100 - 1/1000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Alternative names
Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2
Recommended products
Anti-Sarcomeric Alpha Actinin antibody (ab137346) is a rabbit polyclonal antibody that is used to detect Sarcomeric Alpha Actinin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 40 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACTN2. The exact immunogen used to generate this antibody is proprietary information. Database link P35609
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Sarcomeric Alpha Actinin antibody (ab137346) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Sarcomeric Alpha Actinin?
Anti-Sarcomeric Alpha Actinin (ab137346) specifically detects a band for Sarcomeric Alpha Actinin (UniProt: P35609) at a molecular weight of 103kDa.
Trusted by the scientific community
Anti-Sarcomeric Alpha Actinin (ab137346) was first used in a scientific publication in 2012 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Sarcomeric Alpha Actinin (ab137346) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sarcomeric Alpha Actinin often referred to as alpha-actinin or A-actinin is a protein that functions mechanically as a cross-linker of actin filaments in the sarcomere. It plays a critical role in organizing the actin cytoskeleton providing structural integrity and facilitating muscle contraction. The protein typically has a molecular mass of approximately 100 kDa. It is expressed in a variety of tissues including skeletal and cardiac muscle where it establishes the architecture of the Z-disc by anchoring actin filaments.
- Biological function summary
Sarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.
- Pathways
Alpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.
- Associated diseases and disorders
Mutations or defects in alpha actinin have implications in muscular dystrophy and cardiomyopathy. These conditions often involve disruptions in the muscle's structural framework and mechanical signaling where alpha actinin's interaction with dystrophin becomes significant. Additionally such disorders may showcase altered connectivity between alpha actinin and spectrin further affecting muscle integrity and function in pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (ab137346)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 1/2000. Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (ab137346)
Immunofluorescent analysis of ice-cold methanol fixed (5 minutes) HeLa cells labeling Sarcomeric Alpha Actinin with ab137346 at 1/500 dilution (4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) (keep from light) at 1/2000 dilution (room temperature for 1 hour) (green). Blue: Hoechst 33342 staining.
Blocking: 2.5% BSA/PBS for 30 minutes.
Washing: PBS, 3 x 3 minutes.
Western blot - Anti-Sarcomeric Alpha Actinin antibody (ab137346)
5% SDS-PAGE
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody (ab137346) at 1/1000 dilution
Lane 1: 293T cells at 30 µg
Lane 2: A431 cells at 30 µg
Lane 3: HeLa cells at 30 µg
Lane 4: HepG2 cells at 30 µg
SecondaryAll lanes: HRP conjugated anti-rabbit IgG antibody
Predicted band size: 103 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (ab137346)
Immunohistochemical analysis of paraffin-embedded Human heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 10 μg/ml. Antigen retrieval: EDTA based, pH 8.0, buffer, 15minutes.
Western blot - Anti-Sarcomeric Alpha Actinin antibody (ab137346)
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody (ab137346) at 1/1000 dilution
Lane 1: Neuro2A whole cell lysate/extract at 30 µg
Lane 2: GL261 whole cell lysate/extract at 30 µg
Lane 3: C8D30 whole cell lysate/extract at 30 µg
Lane 4: NIH-3T3 whole cell lysate/extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 103 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (ab137346)
Immunohistochemical analysis of paraffin-embedded rat heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 1/2000. Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
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