AB72592

Anti-Sarcomeric Alpha Actinin antibody

Name: Anti-Sarcomeric Alpha Actinin antibody (ab72592) | Abcam
Brand: Abcam Limited
SKU: AB72592
Rating: 2 (1 reviews)

(1 Review)

(28 Publications)

Rabbit Polyclonal Sarcomeric Alpha Actinin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human ACTN2.

View Alternative Names

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab72592, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

Immunofluorescent analysis of HeLa cells using ab72592, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising peptide.

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Western blot - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

All lanes:

Western blot - Anti-Sarcomeric Alpha Actinin antibody (ab72592) at 1/500 dilution

Lane 1:

Extracts from HeLa cells at 5 µg

Lane 2:

Extracts from HepG2 cells at 5 µg

Lane 3:

Extracts from HUVEC cells at 5 µg

Lane 4:

Extracts from HUVEC cells at 5 µg with immunising peptide

Predicted band size: 103 kDa

Observed band size: 103 kDa,80 kDa,85 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ACTN2. The exact immunogen used to generate this antibody is proprietary information.

P35609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sarcomeric Alpha Actinin often referred to as alpha-actinin or A-actinin is a protein that functions mechanically as a cross-linker of actin filaments in the sarcomere. It plays a critical role in organizing the actin cytoskeleton providing structural integrity and facilitating muscle contraction. The protein typically has a molecular mass of approximately 100 kDa. It is expressed in a variety of tissues including skeletal and cardiac muscle where it establishes the architecture of the Z-disc by anchoring actin filaments.

Biological function summary

Sarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.

Pathways

Alpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.

Mutations or defects in alpha actinin have implications in muscular dystrophy and cardiomyopathy. These conditions often involve disruptions in the muscle's structural framework and mechanical signaling where alpha actinin's interaction with dystrophin becomes significant. Additionally such disorders may showcase altered connectivity between alpha actinin and spectrin further affecting muscle integrity and function in pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

See full target information ACTN2

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 13:e13328 PubMed33751844

2021

Peripheral blood transcriptome profiling enables monitoring disease progression in dystrophic mice and patients.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Signorelli,Mitra Ebrahimpoor,Olga Veth,Kristina Hettne,Nisha Verwey,Raquel García-Rodríguez,Christa L Tanganyika-deWinter,Luz B Lopez Hernandez,Rosa Escobar Cedillo,Benjamín Gómez Díaz,Olafur T Magnusson,Hailiang Mei,Roula Tsonaka,Annemieke Aartsma-Rus,Pietro Spitali

Orphanet journal of rare diseases 15:119 PubMed32448375

2020

Frizzled related protein deficiency impairs muscle strength, gait and calpain 3 levels.

Applications

Unspecified application

Species

Unspecified reactive species

Leire Casas-Fraile,Frederique M Cornelis,Domiziana Costamagna,Anabel Rico,Robin Duelen,Maurilio M Sampaolesi,Adolfo López de Munain,Rik J Lories,Amets Sáenz

Nucleic acid therapeutics 29:92-103 PubMed30672725

2019

Nonclinical Exon Skipping Studies with 2'-O-Methyl Phosphorothioate Antisense Oligonucleotides in mdx and mdx-utrn-/- Mice Inspired by Clinical Trial Results.

Applications

Unspecified application

Species

Unspecified reactive species

Maaike van Putten,Christa Tanganyika-de Winter,Sieto Bosgra,Annemieke Aartsma-Rus

Scientific reports 8:16519 PubMed30410044

2018

Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Gartz,Ashley Darlington,Muhammed Zeeshan Afzal,Jennifer L Strande

Journal of molecular and cellular cardiology 125:29-38 PubMed30336143

2018

Voluntary exercise improves muscle function and does not exacerbate muscle and heart pathology in aged Duchenne muscular dystrophy mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bauke Kogelman,Kayleigh Putker,Margriet Hulsker,Christa Tanganyika-de Winter,Louise van der Weerd,Annemieke Aartsma-Rus,Maaike van Putten

The international journal of biochemistry & cell biology 105:134-143 PubMed30316870

2018

Modulation of alternative splicing of trafficking genes by genome editing reveals functional consequences in muscle biology.

Applications

Unspecified application

Species

Unspecified reactive species

R Eric Blue,Amrita Koushik,Nichlas M Engels,Hannah J Wiedner,Thomas A Cooper,Jimena Giudice

Neuromuscular disorders : NMD 28:427-442 PubMed29631954

2018

Low dystrophin levels are insufficient to normalize the neuromuscular synaptic abnormalities of mdx mice.

Applications

Species

Mouse

Elizabeth M van der Pijl,Maaike van Putten,Erik H Niks,Jan J G M Verschuuren,Annemieke Aartsma-Rus,Jaap J Plomp

PloS one 13:e0193289 PubMed29466448

2018

A dystrophic Duchenne mouse model for testing human antisense oligonucleotides.

Applications

Unspecified application

Species

Unspecified reactive species

Marcel Veltrop,Laura van Vliet,Margriet Hulsker,Jill Claassens,Conny Brouwers,Cor Breukel,Jos van der Kaa,Margot M Linssen,Johan T den Dunnen,Sjef Verbeek,Annemieke Aartsma-Rus,Maaike van Putten

Molecular therapy : the journal of the American Society of Gene Therapy 26:132-147 PubMed29103911

2017

Cyclic Peptides to Improve Delivery and Exon Skipping of Antisense Oligonucleotides in a Mouse Model for Duchenne Muscular Dystrophy.

Applications

Species

Mouse

Silvana M G Jirka,Peter A C 't Hoen,Valeriano Diaz Parillas,Christa L Tanganyika-de Winter,Ruurd C Verheul,Begona Aguilera,Peter C de Visser,Annemieke M Aartsma-Rus

Nature communications 8:1249 PubMed29093487

2017

MicroRNAs promote skeletal muscle differentiation of mesodermal iPSC-derived progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Giacomazzi,Bryan Holvoet,Sander Trenson,Ellen Caluwé,Bojana Kravic,Hanne Grosemans,Álvaro Cortés-Calabuig,Christophe M Deroose,Danny Huylebroeck,Said Hashemolhosseini,Stefan Janssens,Elizabeth McNally,Mattia Quattrocelli,Maurilio Sampaolesi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sarcomeric Alpha Actinin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

Western blot - Anti-Sarcomeric Alpha Actinin antibody (AB72592)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

Product promise