Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (AB257317)

Formalin-fixed, paraffin-embedded human cardiac muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (AB257317)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (AB257317)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

• Protein Array

Supplier Data

Protein Array - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (AB257317)

Analysis of >19,00 full-length human proteins using ab257317.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

• WB

Supplier Data

Western blot - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (AB257317)

All lanes:

Western blot - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (ab257317) at 2 µg/mL

All lanes:

Human skeletal muscle tissue lysate

Predicted band size: 103 kDa

false