Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)

Rabbit Recombinant Monoclonal Sarcomeric Alpha Actinin antibody. Carrier free. Suitable for Dot, IHC-P, ICC/IF, WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (AB317696), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	IHC-P	ICC/IF	WB	Flow Cyt (Intra)	IP
Human	Expected	Not recommended	Tested	Tested	Not recommended	Not recommended
Mouse	Expected	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Expected	Tested	Expected	Tested	Not recommended	Not recommended
Recombinant full length protein - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Target data

See full target information Alpha actinin 2

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Alternative names

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2

Recommended products

Rabbit Recombinant Monoclonal Sarcomeric Alpha Actinin antibody. Carrier free. Suitable for Dot, IHC-P, ICC/IF, WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28776-83
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for human IHC.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab317696 is the carrier-free version of Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Sarcomeric Alpha Actinin often referred to as alpha-actinin or A-actinin is a protein that functions mechanically as a cross-linker of actin filaments in the sarcomere. It plays a critical role in organizing the actin cytoskeleton providing structural integrity and facilitating muscle contraction. The protein typically has a molecular mass of approximately 100 kDa. It is expressed in a variety of tissues including skeletal and cardiac muscle where it establishes the architecture of the Z-disc by anchoring actin filaments.

Biological function summary

Sarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.

Pathways

Alpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.

Associated diseases and disorders

Mutations or defects in alpha actinin have implications in muscular dystrophy and cardiomyopathy. These conditions often involve disruptions in the muscle's structural framework and mechanical signaling where alpha actinin's interaction with dystrophin becomes significant. Additionally such disorders may showcase altered connectivity between alpha actinin and spectrin further affecting muscle integrity and function in pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary cardiomyocyte cells labelling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/500 (1.05 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in mouse primary cardiomyocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-Sarcomeric Alpha Actinin antibody [EA-53] ab9465 Anti-Sarcomeric Alpha Actinin mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

-ve control 2: Anti-Sarcomeric Alpha Actinin antibody [EA-53] ab9465 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary cardiomyocyte cells labelling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/500 (1.05 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in rat primary cardiomyocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-Sarcomeric Alpha Actinin antibody [EA-53] ab9465 Anti-Sarcomeric Alpha Actinin mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

-ve control 2: Anti-Sarcomeric Alpha Actinin antibody [EA-53] ab9465 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.
Dot Blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Dot blot analysis of Sarcomeric Alpha Actinin using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1:1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: His-tagged human ACTN2 recombinant protein

Lane 2: His-tagged human ACTN4 recombinant protein

Lane 3: His-tagged human ACTN1 recombinant protein

Lane 4: His-tagged human ACTN3 recombinant protein
Anti-His tag antibody (1:10000) as total protein control.
This antibody does not cross-react with human ACTN1, human ACTN3 and human ACTN4.
All lanes: Dot Blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695) at 1/1000 dilution
Lane 1: His-tagged human ACTN2 recombinant protein with NFDM/TBST
Lane 2: His-tagged human ACTN4 recombinant protein with NFDM/TBST
Lane 3: His-tagged human ACTN1 recombinant protein with NFDM/TBST
Lane 4: His-tagged human ACTN3 recombinant protein with NFDM/TBST
Secondary
All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat liver. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cardiac muscle. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat skeletal muscle. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse skeletal muscle. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/500 (1.05 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RD (human muscle multinucleated spindle-shaped cell) cells labelling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695 at 1/500 (1.05 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic and membranous staining in RD cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Negative control: liver (PMID:11440986).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695) at 1/1000 dilution
Lane 1: Rat skeletal muscle tissue lysate at 50 µg with NFDM/TBST
Lane 2: Rat heart tissue lysate at 50 µg with NFDM/TBST
Lane 3: Rat liver tissue lysate at 50 µg with NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 103 kDa, 36 kDa
Exposure time: 26s
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Negative control: liver, placenta (PMID:11440986).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 180 seconds, Lanes 3-6: 26 seconds
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695) at 1/1000 dilution
Lane 1: Untreated C2C12 (mouse myoblast) whole cell lysate at 50 µg with NFDM/TBST
Lane 2: C2C12 differentiation to muscle for 5 days whole cell lysate at 50 µg with NFDM/TBST
Lane 3: Mouse skeletal muscle tissue lysate at 50 µg with NFDM/TBST
Lane 4: Mouse heart tissue lysate at 50 µg with NFDM/TBST
Lane 5: Mouse liver tissue lysate at 50 µg with NFDM/TBST
Lane 6: Mouse placenta tissue lysate at 50 µg with NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 103 kDa, 36 kDa
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695, the same antibody clone in a different buffer formulation.
Negative control: liver, pancreas, placenta (PMID:11440986).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] ab317695) at 1/1000 dilution
Lane 1: Human heart tissue lysate at 20 µg with NFDM/TBST
Lane 2: Human skeletal muscle tissue lysate at 50 µg with NFDM/TBST
Lane 3: Human liver tissue lysate at 50 µg with NFDM/TBST
Lane 4: Human pancreas tissue lysate at 50 µg with NFDM/TBST
Lane 5: Human placenta tissue lysate at 50 µg with NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 103 kDa, 36 kDa
Exposure time: 180s