Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)

Rabbit Multiclonal Sarcomeric Alpha Actinin antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	Flow Cyt (Intra)	ICC/IF
Human	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Not recommended	Not recommended	Not recommended
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Alpha actinin 2

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Alternative names

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2

Recommended products

Rabbit Multiclonal Sarcomeric Alpha Actinin antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Purification technique: Affinity purification Protein A
Specificity: This antibody displays limited ACTN3 cross-reactivity in an exogenous setting.
Unsuitable for mosue and rat IP.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab314911 is the carrier-free version of Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910.

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Sarcomeric Alpha Actinin often referred to as alpha-actinin or A-actinin is a protein that functions mechanically as a cross-linker of actin filaments in the sarcomere. It plays a critical role in organizing the actin cytoskeleton providing structural integrity and facilitating muscle contraction. The protein typically has a molecular mass of approximately 100 kDa. It is expressed in a variety of tissues including skeletal and cardiac muscle where it establishes the architecture of the Z-disc by anchoring actin filaments.

Biological function summary

Sarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.

Pathways

Alpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.

Associated diseases and disorders

Mutations or defects in alpha actinin have implications in muscular dystrophy and cardiomyopathy. These conditions often involve disruptions in the muscle's structural framework and mechanical signaling where alpha actinin's interaction with dystrophin becomes significant. Additionally such disorders may showcase altered connectivity between alpha actinin and spectrin further affecting muscle integrity and function in pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody displays limited ACTN3 cross-reactivity in an exogenous setting.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910) at 1/1000 dilution
Lane 1: His-tagged human actn1 recombinant protein at 20 ng
Lane 2: His-tagged human actn2 recombinant protein at 10 ng
Lane 3: His-tagged human actn3 recombinant protein at 20 ng
Lane 4: His-tagged human actn4 recombinant protein at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: nearly no staining on rat kidney. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse kidney. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human kidney. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cardiac muscle. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse skeletal muscle. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human cardiac muscle. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Sarcomeric Alpha Actinin was immunoprecipitated from 0.35 mg RD (human muscle multinucleated spindle-shaped cell) whole cell lysate with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RD (human muscle multinucleated spindle-shaped cell) whole cell lysate
Lane 2: Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 IP in RD (human muscle multinucleated spindle-shaped cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 in RD whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910) at 1/30 dilution
All lanes: RD (human muscle multinucleated spindle-shaped cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 23s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human skeletal muscle. The section was incubated with Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: Kindey.
The identity of the lower MW band at approximately 45kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 103 kDa, 36 kDa
Exposure time: 15s
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (ab314911)
This data was developed using Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The identity of the lower MW band at approximately 45kDa (in lane 3/5) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-5: 4 seconds; Lane 6: 59 seconds
All lanes: Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (Anti-Sarcomeric Alpha Actinin antibody [RM1072] ab314910) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Mouse heart tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
Lane 5: Rat heart tissue lysate at 20 µg
Lane 6: RD (human muscle mtinucleated spindle-shaped cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 103 kDa, 36 kDa