Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free
- BOND RX™ Validated
- RabMAb
- Recombinant
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Rabbit Recombinant Multiclonal Sarcomeric Alpha Actinin antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
View Alternative Names
Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Sarcomeric Alpha Actinin with ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Sarcomeric Alpha Actinin with ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human kidney. The section was incubated with ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin with ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Sarcomeric Alpha Actinin was immunoprecipitated from 0.35 mg RD (human muscle multinucleated spindle-shaped cell) whole cell lysate with ab314910 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314910 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : RD (human muscle multinucleated spindle-shaped cell) whole cell lysate
Lane 2 : ab314910 IP in RD (human muscle multinucleated spindle-shaped cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314910 in RD whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (<a href='/en-us/products/primary-antibodies/sarcomeric-alpha-actinin-antibody-rm1072-ab314910'>ab314910</a>) at 1/30 dilution
All lanes:
RD (human muscle multinucleated spindle-shaped cell) whole cell lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 23s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sarcomeric Alpha Actinin with ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse kidney. The section was incubated with ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sarcomeric Alpha Actinin with ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : nearly no staining on rat kidney. The section was incubated with ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Sarcomeric Alpha Actinin with ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cardiac muscle. The section was incubated with ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Sarcomeric Alpha Actinin with ab314910 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab314910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
- WB
Supplier Data
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Blocking and diluting buffer and concentration : 5% NFDM/TBST
The identity of the lower MW band at approximately 45kDa (in lane 3/5) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time : Lanes 1-5 : 4 seconds; Lane 6 : 59 seconds
All lanes:
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (<a href='/en-us/products/primary-antibodies/sarcomeric-alpha-actinin-antibody-rm1072-ab314910'>ab314910</a>) at 1/1000 dilution
Lane 1:
Human skeletal muscle tissue lysate at 20 µg
Lane 2:
Mouse skeletal muscle tissue lysate at 20 µg
Lane 3:
Mouse heart tissue lysate at 20 µg
Lane 4:
Rat skeletal muscle tissue lysate at 20 µg
Lane 5:
Rat heart tissue lysate at 20 µg
Lane 6:
RD (human muscle mtinucleated spindle-shaped cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 103 kDa,36 kDa
false
- WB
Supplier Data
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Low expression : Kindey.
The identity of the lower MW band at approximately 45kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (<a href='/en-us/products/primary-antibodies/sarcomeric-alpha-actinin-antibody-rm1072-ab314910'>ab314910</a>) at 1/1000 dilution
Lane 1:
Mouse brain tissue lysate at 20 µg
Lane 2:
Mouse heart tissue lysate at 20 µg
Lane 3:
Mouse kidney tissue lysate at 20 µg
Lane 4:
Rat brain tissue lysate at 20 µg
Lane 5:
Rat kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 103 kDa,36 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] - BSA and Azide free (AB314911)
This data was developed using ab314910, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
This antibody displays limited ACTN3 cross-reactivity in an exogenous setting.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-Sarcomeric Alpha Actinin antibody [RM1072] (<a href='/en-us/products/primary-antibodies/sarcomeric-alpha-actinin-antibody-rm1072-ab314910'>ab314910</a>) at 1/1000 dilution
Lane 1:
His-tagged human actn1 recombinant protein at 20 ng
Lane 2:
His-tagged human actn2 recombinant protein at 10 ng
Lane 3:
His-tagged human actn3 recombinant protein at 20 ng
Lane 4:
His-tagged human actn4 recombinant protein at 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 100 kDa
false
Exposure time: 180s
Related conjugates and formulations (1)
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Anti-Sarcomeric Alpha Actinin antibody [RM1072]
Reactivity data
Product details
ab314911 is the carrier-free version of ab314910.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Sarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.
Pathways
Alpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com