Anti-Sarcomeric Alpha Actinin antibody [RP23040128]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [RP23040128] (AB313448)

Immunofluorescent analysis of human skeletal muscle myoblast cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous sarcomeric alpha actin with ab313448 at 2 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of sarcomeric alpha actin (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoskeletal localization of sarcomeric alpha actin. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Sarcomeric Alpha Actinin antibody [RP23040128] (AB313448)

Immunohistochemical analysis of rat heart tissue fixed with ice cold acetone (5 min at -20C) and blocked with 2% BSA for 1 hour labelling Sarcomeric alpha actin with ab313448 at 2 ?g/mL dilution, followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Nuclei (blue) were stained using DAPI, and cytoskeletal F-actin (red) was stained using Rhodamine Phalloidin at 1/300 dilution. Panel a) represents staining with the matched isotype control. Panel b) shows a representative section stained for Sarcomeric alpha actin (green). The images were captured at 20X magnification.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Sarcomeric Alpha Actinin antibody [RP23040128] (AB313448)

Immunohistochemical analysis of Sarcomeric alpha actin in mouse heart tissue fixed with ice cold acetone (5 min at -20C) and blocked with 2% BSA for 1 hour labelling Sarcomeric alpha actin with ab313448 at 2 ?g/mL dilution, followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Nuclei (blue) were stained using DAPI, and cytoskeletal F-actin (red) was stained using Rhodamine Phalloidin at 1/300 dilution. Panel a) represents staining with the matched isotype control. Panel b) shows a representative section stained for Sarcomeric alpha actin (green). The images were captured at 20X magnification.

• WB

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Western blot - Anti-Sarcomeric Alpha Actinin antibody [RP23040128] (AB313448)

All lanes:

Western blot - Anti-Sarcomeric Alpha Actinin antibody [RP23040128] (ab313448) at 1 µg/mL

Lane 1:

Mouse skeletal muscle tissue lysate at 30 µg

Lane 2:

Rat skeletal muscle tissue lysate at 30 µg

Lane 3:

Mouse heart tissue lysate at 30 µg

Lane 4:

Rat Heart tissue lysate at 30 µg

Lane 5:

Mouse Brain tissue lysate at 30 µg

Lane 6:

Rat Brain tissue lysate at 30 µg

Lane 7:

Mouse kidney tissue lysate at 30 µg

Lane 8:

Rat kidney tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

Predicted band size: 103.854 kDa

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