JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB223508

Anti-Sarcosine Oxidase/PSO antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Sarcosine Oxidase/PSO antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PIPOX aa 250-350.

View Alternative Names

LPIPOX, PSO, PIPOX, Peroxisomal sarcosine oxidase, L-pipecolate oxidase, L-pipecolic acid oxidase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcosine Oxidase/PSO antibody (AB223508)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcosine Oxidase/PSO antibody (AB223508)

Paraffin-embedded human liver cancer tissue stained for Sarcosine Oxidase/PSO using ab223508 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcosine Oxidase/PSO antibody (AB223508)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcosine Oxidase/PSO antibody (AB223508)

Paraffin-embedded human tonsil tissue stained for Sarcosine Oxidase/PSO using ab223508 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Sarcosine Oxidase/PSO antibody (AB223508)
  • WB

Supplier Data

Western blot - Anti-Sarcosine Oxidase/PSO antibody (AB223508)

All lanes:

Western blot - Anti-Sarcosine Oxidase/PSO antibody (ab223508) at 1/500 dilution

Lane 1:

Rat kidney lysate

Lane 2:

Rat liver lysate

Lane 3:

Mouse brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 44 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PIPOX aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q9P0Z9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sarcosine Oxidase also referred to as PSO is an enzyme with a known molecular mass of approximately 120 kDa. It functions mechanically as a flavoprotein oxidase. Sarcosine oxidase catalyzes the oxidative demethylation of sarcosine converting it into glycine formaldehyde and hydrogen peroxide. This enzyme is commonly expressed in microbial species including various bacteria like Pseudomonas. Sarcosine oxidase consists of multiple subunits and its active form typically includes both small and large subunits.
Biological function summary

Sarcosine oxidase plays an important role in the one-carbon metabolism specifically involved in glycine and sarcosine degradation pathways. It does not form a part of any known large protein complex but it associates with the biochemical pathway that generates formaldehyde. This pathway is significant for its contribution to the cellular pool of one-carbon units which are important for biosynthetic processes like nucleic acids and certain amino acids synthesis.

Pathways

Sarcosine oxidase is involved in the degradation of glycine and sarcosine pathways. These pathways are parts of the one-carbon metabolism network. The enzyme's activity supports the transfer and utilization of one-carbon units in folate-dependent processes. It connects with related enzymes such as glycine decarboxylase which further regulates the flow of one-carbon compounds within the cell. The importance of these pathways lies in their role in maintaining the cellular methylation status and energy metabolism.

Sarcosine oxidase has been associated with prostate cancer and metabolic disorders like nonketotic hyperglycinemia. Increased levels of sarcosine have been linked to prostate cancer progression suggesting its potential as a biomarker. In these conditions the enzyme's activity or misregulation interplays with proteins like glycine N-methyltransferase which control one-carbon metabolism and cellular methylation reactions. Understanding these connections might offer insights into therapeutic targets for managing relevant disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Metabolizes sarcosine and L-pipecolic acid.
See full target information PIPOX
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0315922 PubMed39813259

2025

3D fluorescence staining and confocal imaging of low amount of intestinal organoids (enteroids): Protocol accessible to all.

Applications

Unspecified application

Species

Unspecified reactive species

Ami Gloria Toulehohoun,Caroline Bouzin,Aurélie Daumerie,Luca Maccioni,Peter Stärkel
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB133745

Anti-PARP4 antibody [EPR8230]

RabMAb|Recombinant

Western blot - Anti-PARP4 antibody [EPR8230] (AB133745)

  • 0
  • 0
Primary Antibodies

AB300075

Anti-TRAF1 antibody [EPR26204-112]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

  • 0
  • 0
Primary Antibodies

AB197808

Anti-ACOX2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACOX2 antibody (AB197808)

  • 0
  • 0
Primary Antibodies

AB155282

Anti-FACL4 antibody [EPR8640]

RabMAb|Recombinant

Western blot - Anti-FACL4 antibody [EPR8640] (AB155282)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com