AB204510

Anti-SARI antibody

Name: Anti-SARI antibody (ab204510) | Abcam
Brand: Abcam Limited
SKU: AB204510

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(5 Publications)

Rabbit Polyclonal SARI antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BATF2 aa 1-50.

View Alternative Names

Basic leucine zipper transcriptional factor ATF-like 2, B-ATF-2, Suppressor of AP-1 regulated by IFN, SARI, BATF2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-SARI antibody (AB204510)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARI antibody (AB204510)

SK-MEL-30 cells stained for SARI (green) using ab204510 at 2 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Paraffin embedded human testis tissue stained for SARI using ab204510 at 1/500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Paraffin embedded human colon tissue stained for SARI using ab204510 at 1/500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Paraffin embedded human kidney tissue stained for SARI using ab204510 at 1/500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Paraffin embedded human Fallopian tube tissue stained for SARI using ab204510 at 1/500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BATF2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8N1L9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARI also known as suppressor of AP-1 regulates apoptosis and inflammation in cells. It is a protein with a molecular mass of around 64 kDa. SARI is mainly expressed in immune cells including macrophages and dendritic cells and also found in epithelial tissues. The protein acts mainly in the cytoplasm where it interacts with other molecules to carry out its functions.

Biological function summary

SARI modulates transcription processes involved in immune responses. It forms part of a regulatory complex that includes other proteins like TRAF3 and TBK1 to influence NF-κB signaling pathways. Through this complex SARI can suppress certain transcription factors thereby regulating gene expression linked to inflammation. This regulation helps maintain balanced immune responses ensuring cells do not overreact to stimuli.

Pathways

SARI interacts with the tumor necrosis factor (TNF) and toll-like receptor (TLR) signaling pathways. In these pathways SARI influences how cells respond to cytokines and infectious agents. It achieves this by interacting with key pathway proteins such as TRAF3 and RIPK1. These interactions allow SARI to mediate the downstream effects ultimately affecting cell survival and immune reactions.

SARI has roles in inflammatory diseases and cancer. In inflammatory diseases such as rheumatoid arthritis SARI influences the activity of inflammation-related proteins like NF-κB which can exacerbate or alleviate symptoms. In cancer SARI has been implicated in regulating tumor growth and metastasis interacting with other cancer-related proteins such as STAT3. These connections make SARI a potential therapeutic target in managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system. Following infection, participates in the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes (By similarity). Selectively suppresses CCN1 transcription and hence blocks the downstream cell proliferation signals produced by CCN1 and inhibits CCN1-induced anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma. Possibly acts by interfering with AP-1 binding to CCN1 promoter.

See full target information BATF2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 22:100-116 PubMed35949109

2022

m6A modification-mediated BATF2 suppresses metastasis and angiogenesis of tongue squamous cell carcinoma through inhibiting VEGFA.

Applications

Unspecified application

Species

Unspecified reactive species

Haojie Wen,Jinyong Tang,Yi Cui,Minhua Hou,Juan Zhou

Pathology oncology research : POR 28:1610273 PubMed35369571

2022

RING-Finger Protein 6 promotes Drug Resistance in Retinoblastoma JAK2/STAT3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Chai,Shoufeng Jiao,Xin Peng,Qiang Gan,Leifeng Chen,Xiaolu Hu,Liang Hao,Shouhua Zhang,Qiang Tao

Frontiers in molecular biosciences 8:724373 PubMed34778372

2021

Diagnostic and Prognostic Potential of Circulating and Tissue BATF2 in Nasopharyngeal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaolei Cui,Yingying Lin,Dan Hu,Jing Wu,Wei Peng,Yan Chen

BMC cancer 21:1062 PubMed34565331

2021

Expression and clinical implications of basic leucine zipper ATF-like transcription factor 2 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Lin,Xusheng Zhou,Wei Peng,Jing Wu,Xiufeng Wu,Yan Chen,Zhaolei Cui

The Journal of dermatology 47:e372-e373 PubMed32696470

2020

BATF2 expression as a novel marker for invasive phenotype in malignant melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hidemi Tagawa,Hisashi Kanemaru,Toshihiro Kimura,Haruka Kuriyama,Soichiro Sawamura,Ikko Kajihara,Azusa Miyashita,Jun Aoi,Satoshi Fukushima,Hironobu Ihn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SARI antibody

Immunocytochemistry/ Immunofluorescence - Anti-SARI antibody (AB204510)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARI antibody (AB204510)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise