Skip to main content

Rabbit Polyclonal SARM antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SARM1.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SARM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q6SZW1
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Rat
Predicted
Predicted
Rhesus monkey
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Rat, Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Rat, Rhesus monkey

Dilution info

-

Notes

-

Target data

Function

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed:25908823, PubMed:27671644, PubMed:28334607). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed:15123841, PubMed:16964262, PubMed:20306472, PubMed:25908823). Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction (PubMed:25908823, PubMed:28334607, PubMed:30333228, PubMed:31128467, PubMed:31439793, PubMed:32049506, PubMed:32828421, PubMed:31439792, PubMed:33053563). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (PubMed:29395922). Can activate neuronal cell death in response to stress (PubMed:20306472). Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity). Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PubMed:16964262).

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Polyclonal SARM antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SARM1.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SARM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q6SZW1
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

SARM engages in processes that are important to maintaining neurological health and regulating immune responses. It serves as a part of the innate immunity complex where it contributes to the regulation of inflammatory responses. SARM can also influence mitochondrial function indicating it plays a significant role in cellular energy management and apoptosis control. These multifaceted functions highlight its engagement in complex and dynamic cellular processes.

Activity summary

The sterile alpha and HEAT/Armadillo motif-containing protein commonly known as SARM is a member of the death domain superfamily. It has a molecular mass of approximately 68 kDa. SARM is expressed in the nervous system and various immune cells. Mechanically SARM functions as an adaptor protein involved in signaling pathways related to immunity and neuroprotection. It primarily modulates signaling cascades by interacting with other key proteins within the cellular environment.

Pathways

SARM plays an important role in the toll-like receptor (TLR) signaling pathway and mitochondrial apoptosis pathway. SARM interacts closely with TLRs to modulate immune responses particularly impacting the production of type I interferons. In the mitochondrial apoptosis pathway SARM relates to proteins like TRAF6 impacting cell death and survival. Its involvement in these pathways reflects its essential function in controlling cellular stress responses.

Associated diseases and disorders

SARM has been implicated in neurological diseases such as Alzheimer's disease and infectious diseases such as viral encephalitis. In Alzheimer's disease SARM interacts with other proteins like amyloid precursor protein (APP) modulating pathways that may exacerbate neurodegeneration. In viral encephalitis SARM mediates immune responses providing a link to immune-related proteins like MyD88 which contribute to neuronal damage during infection. This makes SARM a target of interest for therapeutic interventions in both neurological and infectious diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-SARM antibody (ab226930), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SARM antibody (ab226930)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling SARM with ab184257 at 1/500 dilution (Green). Nuclear counterstain is Fluoroshield with DAPI (blue).

  • Western blot - Anti-SARM antibody (ab226930), expandable thumbnail

    Western blot - Anti-SARM antibody (ab226930)

    All lanes: Western blot - Anti-SARM antibody (AB226930) at 1/1000 dilution

    All lanes: 293T whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 79 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com