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AB309195

Anti-SARM1 antibody [EPR24834-80]

  • BOND RX™ Validated
  • 20ul selling size
  • Recombinant
  • KO Validated
  • Advanced Validation
  • RabMAb
  • What is this?

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(1 Review)

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(0 Publication)

Anti-SARM1 antibody [EPR24834-80] (ab309195) is a rabbit monoclonal antibody detecting SARM in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, mIHC. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Kiaa0524, Sarm1, NAD(+) hydrolase SARM1, NADase SARM1, NADP(+) hydrolase SARM1, Sterile alpha and TIR motif-containing protein 1

17 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling SARM1 with ab309195 at 1/50 (9.64 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line, and showing no staining in HaCaTcell line.Negative control : HaCaT.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IP

Supplier Data

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (AB309195)

SARM1 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab309195 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309195 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate Lane 2 : ab309195 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309195 in SH-SY5Y whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/30 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse heart (fresh) tissue labeling SARM1 with ab309195 at 1/50 (9.64 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse heart (PMID : 21555464). The nuclear counterstain was DAPI (Blue). The section was incubated with ab309195 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SARM1 with ab309195 at 1/500 (0.964 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab309195 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SARM1 with ab309195 at 1/500 (0.964 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID : 21555464). The section was incubated with ab309195 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labeling SARM1 with ab309195 at 1/50 (9.64 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309195 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh) tissue labeling SARM1 with ab309195 at 1/50 (9.64 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309195 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling SARM1 with ab309195 at 1/50 (9.64 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on rat heart (PMID : 21555464). The nuclear counterstain was DAPI (Blue). The section was incubated with ab309195 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling SARM1 with ab309195 at 1/500 (0.964 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on mouse cardiac muscle. The section was incubated with ab309195 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Multiplex immunohistochemistry - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse colon tissue staining GSDMC2 + GSDMC3 with ab229896 at a 1 : 2000 (0.25 ug/ml) dilution; SARM1 with ab309195 at 1 : 500 (0.964 ug/ml) dilution and MUC2 with ab272692 at 1 : 2000 (0.252 ug/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-SARM1 (green; Opal™520), anti-MUC2 (gray; Opal™570) and anti-GSDMC2/3 (magenta; Opal™690)on mouse colon.
Panel B : anti-SARM1 staining myenteric nerve plexus in mouse colon.
Panel C : anti-MUC2 staining goblet cells in mouse colon.
Panel D : anti-GSDMC2/3 staining epithelium in mouse colon.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab309195, ab272692 and ab229896 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IP

Supplier Data

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (AB309195)

SARM1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab309195 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309195 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate Lane 2 : ab309195 IP in Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309195 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/30 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • IP

Supplier Data

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (AB309195)

SARM1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab309195 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309195 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat brain tissue lysate Lane 2 : ab309195 IP in Rat brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309195 in rat brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/30 dilution

All lanes:

Rat brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • WB

Supplier Data

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : HaCaT, EL4, mouse heart (PMID : 21555464). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HaCaT (human skin keratinocyte) whole cell lysate at 20 µg

Lane 3:

EL4 (mouse lymphoma t lymphocyte) whole cell lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse heart tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • WB

Supplier Data

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 180 seconds

All lanes:

Western blot - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Rat spinal cord tissue lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 2 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • WB

Lab

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Sarm1-KO homozygous mice (Strain ID : T014509).

All lanes:

Western blot - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/1000 dilution

Lane 1:

Wild-type mouse hippocampus tissue lysate (male case1) at 20 µg

Lane 2:

Sarm1 knockout mouse hippocampus tissue lysate (male case1 at 20 µg

Lane 3:

Sarm1 knockout mouse hippocampus tissue lysate (male case2) at 20 µg

Lane 4:

Wild-type mouse hippocampus tissue lysate (male case2) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

false

Exposure time: 180s

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • WB

Lab

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Sarm1-KO homozygous mice (Strain ID : T014509).

All lanes:

Western blot - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/1000 dilution

Lane 1:

Wild-type mouse brain tissue lysate (male) at 20 µg

Lane 2:

Sarm1 knockout mouse brain tissue lysate (male case1) at 20 µg

Lane 3:

Sarm1 knockout mouse brain tissue lysate (male case2) at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)
  • WB

Supplier Data

Western blot - Anti-SARM1 antibody [EPR24834-80] (AB309195)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Wild-type and KO lysates were kindly provided by Dr. Xiaodong Wang, NIBS. Performed under reducing conditions. In Western blot, ab309195 was shown to bind specifically to SARM1. Target of interest was observed at 70 kDa in wild-type mouse brain tissue lysate (lane 1) with no signal observed at this size in SARM1 knockout mouse brain tissue lysate (lane 2). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200,000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SARM1 antibody [EPR24834-80] (ab309195) at 1/1000 dilution

Lane 1:

Wild-type mouse brain tissue lysate at 20 µg

Lane 2:

SARM1 knockout mouse brain tissue lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

EL4 (mouse lymphoma t lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-SARM1 antibody [EPR24834-80] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24834-80

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB, mIHC, IHC-Fr, IP, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

In IHC-P we observe some non-specific filament-like staining.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-SARM1 antibody [EPR24834-80] (ab309195) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-SARM1 antibody [EPR24834-80] (ab309195) has been confirmed by Western blot testing in Sarm1 Knockout Mouse brain samples.

Other related products
We have a range of other formats of antibody clone [EPR24834-80] also available for your convenience: ab309195, Carrier free - ab309196

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The sterile alpha and HEAT/Armadillo motif-containing protein commonly known as SARM is a member of the death domain superfamily. It has a molecular mass of approximately 68 kDa. SARM is expressed in the nervous system and various immune cells. Mechanically SARM functions as an adaptor protein involved in signaling pathways related to immunity and neuroprotection. It primarily modulates signaling cascades by interacting with other key proteins within the cellular environment.
Biological function summary

SARM engages in processes that are important to maintaining neurological health and regulating immune responses. It serves as a part of the innate immunity complex where it contributes to the regulation of inflammatory responses. SARM can also influence mitochondrial function indicating it plays a significant role in cellular energy management and apoptosis control. These multifaceted functions highlight its engagement in complex and dynamic cellular processes.

Pathways

SARM plays an important role in the toll-like receptor (TLR) signaling pathway and mitochondrial apoptosis pathway. SARM interacts closely with TLRs to modulate immune responses particularly impacting the production of type I interferons. In the mitochondrial apoptosis pathway SARM relates to proteins like TRAF6 impacting cell death and survival. Its involvement in these pathways reflects its essential function in controlling cellular stress responses.

SARM has been implicated in neurological diseases such as Alzheimer's disease and infectious diseases such as viral encephalitis. In Alzheimer's disease SARM interacts with other proteins like amyloid precursor protein (APP) modulating pathways that may exacerbate neurodegeneration. In viral encephalitis SARM mediates immune responses providing a link to immune-related proteins like MyD88 which contribute to neuronal damage during infection. This makes SARM a target of interest for therapeutic interventions in both neurological and infectious diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed : 25818290, PubMed : 26686637, PubMed : 27735788, PubMed : 32312889). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed : 21555464, PubMed : 22678360, PubMed : 25818290, PubMed : 26423149, PubMed : 26686637). Wallerian degeneration is triggered by NAD(+) depletion : in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction (PubMed : 28334607). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (By similarity). Can activate neuronal cell death in response to stress (PubMed : 19587044). Regulates dendritic arborization through the MAPK4-JNK pathway (PubMed : 17724133, PubMed : 21555464). Involved in innate immune response : inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PubMed : 21555464).
See full target information Sarm1
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