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AB307496

Anti-SARM1 antibody [HL1066]

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Rabbit Recombinant Monoclonal SARM antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SARM1.

View Alternative Names

KIAA0524, SAMD2, SARM, SARM1, NAD(+) hydrolase SARM1, NADase SARM1, hSARM1, NADP(+) hydrolase SARM1, Sterile alpha and Armadillo repeat protein, Sterile alpha and TIR motif-containing protein 1, Sterile alpha motif domain-containing protein 2, Tir-1 homolog, MyD88-5, SAM domain-containing protein 2, HsTIR

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARM1 antibody [HL1066] (AB307496)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARM1 antibody [HL1066] (AB307496)

Immunofluorescence analysis of 4% paraformaldehyde fixed SH-SY-5Y cells labelling SARM1 protein using ab307496 at a 1/500 dilution at RT for 15 min (Green). alpha Tubulin, a cytoskeleton marker was stained with anti-alpha Tubulin antibody at a 1/1000 dilution (Red). The nuclear counter stain is DAPI (Blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1066

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SARM1. The exact immunogen used to generate this antibody is proprietary information.

Q6SZW1

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The sterile alpha and HEAT/Armadillo motif-containing protein commonly known as SARM is a member of the death domain superfamily. It has a molecular mass of approximately 68 kDa. SARM is expressed in the nervous system and various immune cells. Mechanically SARM functions as an adaptor protein involved in signaling pathways related to immunity and neuroprotection. It primarily modulates signaling cascades by interacting with other key proteins within the cellular environment.
Biological function summary

SARM engages in processes that are important to maintaining neurological health and regulating immune responses. It serves as a part of the innate immunity complex where it contributes to the regulation of inflammatory responses. SARM can also influence mitochondrial function indicating it plays a significant role in cellular energy management and apoptosis control. These multifaceted functions highlight its engagement in complex and dynamic cellular processes.

Pathways

SARM plays an important role in the toll-like receptor (TLR) signaling pathway and mitochondrial apoptosis pathway. SARM interacts closely with TLRs to modulate immune responses particularly impacting the production of type I interferons. In the mitochondrial apoptosis pathway SARM relates to proteins like TRAF6 impacting cell death and survival. Its involvement in these pathways reflects its essential function in controlling cellular stress responses.

SARM has been implicated in neurological diseases such as Alzheimer's disease and infectious diseases such as viral encephalitis. In Alzheimer's disease SARM interacts with other proteins like amyloid precursor protein (APP) modulating pathways that may exacerbate neurodegeneration. In viral encephalitis SARM mediates immune responses providing a link to immune-related proteins like MyD88 which contribute to neuronal damage during infection. This makes SARM a target of interest for therapeutic interventions in both neurological and infectious diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed : 25908823, PubMed : 27671644, PubMed : 28334607). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed : 15123841, PubMed : 16964262, PubMed : 20306472, PubMed : 25908823). Wallerian degeneration is triggered by NAD(+) depletion : in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction (PubMed : 25908823, PubMed : 28334607, PubMed : 30333228, PubMed : 31128467, PubMed : 31439792, PubMed : 31439793, PubMed : 32049506, PubMed : 32828421, PubMed : 33053563). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (PubMed : 29395922). Can activate neuronal cell death in response to stress (PubMed : 20306472). Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity). Involved in innate immune response : inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PubMed : 16964262).
See full target information SARM1
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Product promise

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For full details, please see our Terms & Conditions

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