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AB313846

Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] - BSA and Azide free

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Rabbit Recombinant Monoclonal SARS-CoV-2 nsp5 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell line - SARS-CoV-2 samples.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] - BSA and Azide free (AB313846)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] - BSA and Azide free (AB313846)

Immunofluorescent analysis of mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling SARS-CoV-2 (COVID-19) 3CLpro (nsp5) using ab313846 at a 1/500 dilution (green). Blue : Hoechst 33342. Cells were fixed in 4% paraformaldehyde at RT for 15 mins.

Western blot - Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] - BSA and Azide free (AB313846)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] - BSA and Azide free (AB313846)

All lanes:

Western blot - Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] - BSA and Azide free (ab313846) at 1/5000 dilution

Lane 1:

Whole cell extracts from non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells

Lane 2:

Whole cell extracts from transfected HEK-293T cells

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1367

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 3CLpro also known as 3C-like protease or nsp5 is a protease enzyme with a critical role in the viral life cycle. It has an estimated mass of about 33.8 kDa. It performs its function primarily in the cytoplasm of the host cell. This protease cleaves the viral polyprotein at specific sites to generate functional proteins necessary for the viral replication and transcription process. It consists of a catalytic dyad made up of cysteine and histidine which aids in the hydrolysis of peptide bonds.
Biological function summary

The 3CLpro ensures the maturation of non-structural proteins necessary for SARS-CoV-2 replication. It is part of a larger polyprotein complex that the virus produces upon infection. The 3CLpro processes this polyprotein to release various functional viral proteins that aid in RNA synthesis and modification. It is a target for antiviral drug development due to its importance in viral propagation and lack of homologous human proteases reducing the likelihood of off-target effects.

Pathways

The function of 3CLpro is integral in the viral replication pathway of SARS-CoV-2. Within this pathway it interacts with viral RNA-dependent RNA polymerase essential for transcribing and replicating the viral genome. Another significant connection occurs with non-structural protein 8 (nsp8) where 3CLpro aids by cleaving the polyprotein to release the mature form of this accessory protein facilitating RNA replication.

SARS-CoV-2 3CLpro's function targets COVID-19 caused by the novel coronavirus. Its proteolytic activity is directly linked to the pathogenesis of COVID-19 as it enables viral replication within host cells. In this context 3CLpro is associated with other viral proteins such as nsp12 the main RNA polymerase critical for the replication process thereby representing a potential therapeutic target to disrupt viral life cycle and mitigate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 nsp5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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