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Rabbit Recombinant Monoclonal VEMP antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line samples. Immunogen corresponding to Synthetic Peptide within SARS-CoV-2 E.

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Images

Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371), expandable thumbnail
  • Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within SARS-CoV-2 E. The exact immunogen used to generate this antibody is proprietary information. Database link P0DTC4

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Transfected cell line
Tested
Tested

Tested
Tested

Species
Transfected cell line
Dilution info
1/500
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/1000
Notes

-

Target data

Function

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed:33229438). May act by slowing down the cell secretory pathway (PubMed:33229438). May interfere with tight-junction stability by interacting with host MPP5. This would result in disruption of epithelial barriers, thereby amplifying inflammatory processes (PubMed:32891874).

Alternative names

4, E, Envelope small membrane protein, sM protein

Recommended products

Rabbit Recombinant Monoclonal VEMP antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line samples. Immunogen corresponding to Synthetic Peptide within SARS-CoV-2 E.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within SARS-CoV-2 E. The exact immunogen used to generate this antibody is proprietary information. Database link P0DTC4
Carrier free
Yes
Clone number
HL1443
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371), expandable thumbnail

    Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] (ab308371) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371) at 1/1000 dilution

    Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg

    Lane 2: Transfected (+) 293T whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371), expandable thumbnail

    Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nipah virus Nucleoprotein antibody [HL1436] (ab308371) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371) at 1/5000 dilution

    Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg

    Lane 2: Transfected (+) 293T whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371)

    For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 Envelope using ab308371 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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