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AB308371

Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443]

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(1 Publication)

Rabbit Recombinant Monoclonal VEMP antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2 samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within SARS-CoV-2 E.

View Alternative Names

4, E, Envelope small membrane protein, sM protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 Envelope using ab308371 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371)
  • WB

Supplier Data

Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (ab308371) at 1/1000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged SARS CoV-2 Envelop protein transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1443

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within SARS-CoV-2 E. The exact immunogen used to generate this antibody is proprietary information.

P0DTC4

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Complementary Products

Primary Antibodies

AB308415

Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088] (AB308415)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB48949

Recombinant SARS E protein (Coronavirus)

  • 0
  • 0
Proteins & Peptides

AB273064

Human coronavirus SARS-CoV-2 envelope protein peptide

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV envelope protein also known as E protein is a small membrane protein with a mass of approximately 9.6 kDa. It is a structural component of the SARS coronavirus and integrates into the viral envelope. This protein expresses primarily in the membranes of infected host cells contributing to virion assembly budding and release. The E protein acts as an ion channel and facilitates virus-to-host cell interaction which is critical during the coronavirus life cycle.
Biological function summary

The E protein functions within virus replication and pathogenesis. It forms part of the viral assembly complex interacting with the membrane (M) protein and nucleocapsid (N) protein during virion maturation. The E protein impacts the host cell’s cellular pathways by altering membrane permeability and modulating host cell stress responses. Additionally its ion channel activity contributes to virus-host cell interactions and is believed to play a role in immune response evasion.

Pathways

The E protein participates in the coronavirus replication and assembly process. It is involved in the virion assembly pathway and interacts with other viral proteins like the M and N proteins to facilitate this process. The E protein's role in the modification of the host cell’s Golgi apparatus assists in viral budding and release an essential part of the coronavirus replication cycle that is important for efficient viral propagation.

The E protein has significance in the pathogenesis of severe acute respiratory syndrome (SARS) and its role in coronavirus infections. Its interaction with other viral proteins like the structural M protein contributes to the inflammatory responses seen in SARS-CoV infections. Additionally due to its ion channel activity the E protein could influence respiratory complications by affecting cellular stress and apoptosis pathways associated with SARS-CoV related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed : 33229438). May act by slowing down the cell secretory pathway (PubMed : 33229438). May interfere with tight-junction stability by interacting with host MPP5. This would result in disruption of epithelial barriers, thereby amplifying inflammatory processes (PubMed : 32891874).
See full target information E
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406211 PubMed39301916

2024

Lung-Targeted Lipid Nanoparticle-Delivered siUSP33 Attenuates SARS-CoV-2 Replication and Virulence by Promoting Envelope Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzheng Zhou,Yujie Liao,Lujie Fan,Xiafei Wei,Qiang Huang,Chuwei Yang,Wei Feng,Yezi Wu,Xiang Gao,Xiaotong Shen,Jian Zhou,Zanxian Xia,Zheng Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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