AB308415

Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal VEMP antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2 samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within SARS-CoV-2 E.

View Alternative Names

4, E, Envelope small membrane protein, sM protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088] (AB308415)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088] (AB308415)

For immunofluorescence analysis, HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 (COVID 19) Membrane using ab308415 at 1/2000 dilution (green). Blue : Fluoroshield with DAPI.

Supplier Data

Western blot - Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088] (AB308415)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Membrane antibody [HL1088] (ab308415) diluted at 1/20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088] (ab308415) at 1/20000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

Transfected (+) 293T unboiled whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1088

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within SARS-CoV-2 E. The exact immunogen used to generate this antibody is proprietary information.

P0DTC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/2000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 M protein also known as membrane protein plays an important mechanical role in the virus's life cycle. It is a small protein with a mass of approximately 25 to 30 kilodaltons. It is expressed on the surface of the virus envelope and is highly conserved among coronaviruses. The M protein is integral to assembling new viral particles and facilitates the virus's ability to maintain its structure. It influences the shape and integrity of the viral envelope by interacting with other viral proteins such as the spike protein and the envelope protein.

Biological function summary

The SARS-CoV-2 membrane protein plays a significant role in the viral infection process. It functions to orchestrate the assembly of new virions by localizing at the endoplasmic reticulum-Golgi intermediate compartment (ERGIC). The M protein often interacts with the nucleocapsid (N) protein forming a complex essential for packaging viral RNA. This partnership ensures the virus efficiently assembles and buds from the host cell aiding in the sufficient production of infectious particles.

Pathways

The SARS-CoV-2 M protein stands central in viral pathogenesis. It participates in the formation of the viral replication complex within the host cell. The protein interacts with the spike (S) protein facilitating viral entry into the host by enabling the fusion of the viral and cellular membranes through its role in the ERGIC. Additionally the M protein is involved in pathways related to modulating host immune responses often dampening interferon signaling which is important for the host's initial defense against viral infections.

The SARS-CoV-2 membrane protein is intimately linked to COVID-19 the disease caused by the virus. This protein along with other structural proteins like the envelope (E) protein contributes to the pathogenicity and virulence of the virus impacting disease severity. The M protein also has associations with immune evasion strategies employed by the virus which may lead to more severe respiratory complications in infected patients. Understanding its interactions with the host immune system and other viral proteins sheds light on developing targeted therapeutics and vaccines against COVID-19.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed : 33229438). May act by slowing down the cell secretory pathway (PubMed : 33229438). May interfere with tight-junction stability by interacting with host MPP5. This would result in disruption of epithelial barriers, thereby amplifying inflammatory processes (PubMed : 32891874).

See full target information E

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406211 PubMed39301916

2024

Lung-Targeted Lipid Nanoparticle-Delivered siUSP33 Attenuates SARS-CoV-2 Replication and Virulence by Promoting Envelope Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzheng Zhou,Yujie Liao,Lujie Fan,Xiafei Wei,Qiang Huang,Chuwei Yang,Wei Feng,Yezi Wu,Xiang Gao,Xiaotong Shen,Jian Zhou,Zanxian Xia,Zheng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088]

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088] (AB308415)

Western blot - Anti-SARS CoV 2 (COVID 19) Membrane antibody [HL1088] (AB308415)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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