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AB308368

Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435]

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Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples. Immunogen corresponding to Synthetic Peptide within SARS-CoV-2 rep.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435] (AB308368)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435] (AB308368)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 nsp1 using ab308368 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435] (AB308368)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435] (AB308368)

Immunohistochemical analysis of SARS-CoV-2 (COVID-19) nsp1 transfected HEK293T labeling SARS CoV 2 COVID 19 nsp1 using ab308368 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435] (AB308368)
  • WB

Supplier Data

Western blot - Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435] (AB308368)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp1 antibody [HL1435] (ab308368) diluted at 1/10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SARS CoV 2 COVID 19 nsp1 antibody [HL1435] (ab308368) at 1/10000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1435

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within SARS-CoV-2 rep. The exact immunogen used to generate this antibody is proprietary information.

P0DTD1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp1 also known as non-structural protein 1 is a viral protein with a molecular mass of around 19.8 kDa. It is produced by the SARS-CoV-2 virus the agent responsible for the COVID-19 pandemic. Nsp1 is expressed in the cytoplasm of infected host cells. It is important for the virus as it plays a significant role in the regulation of host cell processes making it an interesting target for antiviral research.
Biological function summary

This non-structural protein inhibits host cellular gene expression. Nsp1 disrupts the host's mRNA translation machinery by directly interacting with the ribosomal 40S subunit. This interference enables the virus to suppress the cell's immune response aiding viral replication and assembly. Nsp1 does not form part of a large complex but functions independently to achieve its suppressive effects.

Pathways

Nsp1's function aligns with pathways related to host translation processes and immune evasion tactics. It acts to inhibit the IFN-mediated signaling pathway preventing the usual anti-viral response of the cell. By blocking mRNA binding to the ribosome nsp1 contributes to the shutdown of host protein synthesis which allows virus replication to proceed without host interference. It is closely connected to other viral non-structural proteins that initiate viral replication and assembly within the host.

Nsp1 is directly linked to COVID-19 due to its origin from the SARS-CoV-2 virus. Nsp1's disruption of immune response pathways may contribute to the severity of the disease's impact. It also has indirect relationships with various inflammatory responses due to its immune evasion capability. Through nsp1's action of inhibiting host cell processes it holds potential connections with other proteins involved in immune signaling pathways contributing to investigations into therapeutic interventions against COVID-19.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 PLpro
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com