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AB308421

Anti-SARS CoV 2 (COVID 19) nsp10 antibody [HL1379]

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Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2 samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to SARS-CoV-2 rep.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) nsp10 antibody [HL1379] (AB308421)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) nsp10 antibody [HL1379] (AB308421)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 (COVID 19) nsp10 using ab308421 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-SARS CoV 2 (COVID 19) nsp10 antibody [HL1379] (AB308421)
  • WB

Supplier Data

Western blot - Anti-SARS CoV 2 (COVID 19) nsp10 antibody [HL1379] (AB308421)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp10 antibody [HL1379] (ab308421) diluted at 1/10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SARS CoV 2 (COVID 19) nsp10 antibody [HL1379] (ab308421) at 1/10000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1379

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to SARS-CoV-2 rep.

P0DTD1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp10 also known as non-structural protein 10 serves as a cofactor essential for the viral life cycle. It is about 15 kDa in mass and is expressed within infected host cells where the virus replicates. Mechanically nsp10 facilitates the formation of nsp10-nsp14 and nsp10-nsp16 complexes enhancing the exonuclease and methyltransferase activities of its partners. This function supports the viral RNA proofreading and mRNA cap methylation processes contributing to the clinical pathogenicity of the virus.
Biological function summary

Nsp10 plays a role in stabilizing nsp14 and nsp16 to help them perform their enzymatic functions effectively. These interactions involve complex formations required for maintaining the integrity of viral RNA synthesis. By coordinating with nsp14 nsp10 ensures the accurate proofreading of RNA to protect against mutations. In association with nsp16 it assists in mRNA cap methylation which is pivotal for evading host immune responses.

Pathways

The interaction between nsp10 and its partner proteins integrate it into important viral replication and host immune evasion pathways. These pathways are critical for the SARS-CoV-2 lifecycle facilitating replication and transcription mechanisms. Through its role with nsp14 nsp10 contributes to the exonuclease pathway important for RNA error correction. Its association with nsp16 is significant in the methylation pathway preventing premature degradation of viral mRNA by the host cell’s immune system.

Nsp10 directly influences the progression of COVID-19 the disease caused by SARS-CoV-2. Its interactions with nsp14 and nsp16 affect the virus's ability to replicate efficiently and avoid detection by host defenses which can influence the severity of the disease. Understanding the dynamics of nsp10's interactions offers potential therapeutic targets for treating COVID-19 and managing the viral replication process to curb the disease's impact.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 PLpro
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com