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AB313847

Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free

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Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell line - SARS-CoV-2 samples.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free (AB313847)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free (AB313847)

Immunofluorescent analysis of mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling SARS-CoV-2 (COVID-19) nsp10 using ab313847 at a 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Cells were fixed in 4% paraformaldehyde at RT for 15 mins.

Western blot - Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free (AB313847)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free (AB313847)

All lanes:

Western blot - Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free (ab313847) at 1/5000 dilution

Lane 1:

Whole cell extracts from non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells at 30 µg

Lane 2:

Whole cell extracts from transfected HEK-293T cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1381

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nonstructural protein 10 (nsp10) also known as growth factor-like protein 1 acts mechanically as a cofactor to enhance the activity of nsp14 and nsp16. Nsp10 with a molecular mass of approximately 14 kDa facilitates the methylation of viral RNA which is important for viral replication and evasion of the host immune response. This protein expresses in the host cells infected by the virus particularly in the cytoplasm where viral replication occurs.
Biological function summary

The interaction of nsp10 with other viral proteins is essential for the virus's lifecycle. Nsp10 forms a complex with nsp14 a proofreading exonuclease and nsp16 a 2'-O-methyltransferase. This complex ensures the integrity and stability of viral RNA by capping and proofreading protecting it from host immune detection. By forming this complex nsp10 supports the efficient replication and transcription of viral RNA an important process for SARS-CoV-2 infection and propagation in the host.

Pathways

Nsp10 participates in viral replication and host immune evasion strategies. It is integral to the RNA capping and modification pathways ensuring that SARS-CoV-2 RNA is efficiently transcribed and translated. This protein interacts particularly with nsp14 and nsp16 aiding in the proper assembly of the replication-transcription complex. These interactions allow the virus to maintain its genomic fidelity and to avoid the immune response important for successful infection.

Nsp10 has a significant role in the pathogenesis of COVID-19 the disease caused by SARS-CoV-2. It influences the severity of the disease by facilitating effective viral replication and immune evasion strategies. Additionally research indicates links between nsp10 and other respiratory diseases highlighting its role in viral pathogenesis. The interaction of nsp10 with proteins like nsp14 positions it as a potential target for therapeutic intervention to disrupt its function and combat COVID-19 effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 nsp10
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com