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AB308367

Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415]

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Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415] (AB308367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415] (AB308367)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 nsp12 using ab308367 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415] (AB308367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415] (AB308367)

Immunohistochemical analysis of SARS-CoV-2 (COVID-19) nsp12 transfected HEK293T cell FFPE Cell Pellet Block labeling SARS CoV 2 COVID 19 nsp12 using ab308367 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415] (AB308367)
  • WB

Supplier Data

Western blot - Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415] (AB308367)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 antibody [HL1415] (ab308367) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SARS CoV 2 COVID 19 nsp12 antibody [HL1415] (ab308367) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1415

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp12 also known as nonstructural protein 12 functions as an RNA-dependent RNA polymerase (RdRp) important for viral RNA genome replication. This protein weighs approximately 106 kilodaltons and derives from the SARS-CoV-2 pathogen the virus responsible for COVID-19. Nsp12 integral to the replication complex is expressed primarily in the host cell's cytoplasm during coronavirus infection. Together with other nonstructural proteins it directs the replication and transcription of the viral genome thereby playing an important role in the viral life cycle.
Biological function summary

Nonstructural protein 12 associates with cofactors nsp7 and nsp8 to form a functional polymerase complex essential for replicating the viral RNA genome. This complex efficiently extends RNA strands from RNA templates using the 3' end as a starting point. Nsp12 contains a conserved polymerase domain which ensures the fidelity of replication. It performs extensive proofreading alongside overlapping proteins like nsp14 which provide exonuclease activity to correct errors sustaining the propagation of the virus within the host.

Pathways

The nsp12 protein directly interacts with mechanisms like viral RNA replication and viral mRNA synthesis. It holds a central position in the coronavirus replication-transcription complex working closely with other nonstructural proteins such as nsp7 and nsp8. These interactions enable the synthesis of full-length genomic RNA and shorter subgenomic RNAs critical for translating viral structural proteins therefore facilitating the continuous cycle of infection and propagation.

Nsp12's activity directly relates to COVID-19 pathogenesis making it a significant target for antiviral treatments. The inhibition of nsp12 through therapeutic agents can effectively disrupt viral RNA synthesis curbing the replication of SARS-CoV-2. Remdesivir a nucleotide analog drug that targets the RdRp activity of nsp12 exemplifies this strategy specifically impeding the SARS-CoV-2 replication process. Additionally variations in nsp12 and its associated protein interactions influence the severity and progression of coronavirus infections in affected individuals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 PLpro
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com