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AB308423

Anti-SARS CoV 2 (COVID 19) nsp15 antibody [HL1446]

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Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.
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Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) nsp15 antibody [HL1446] (AB308423)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) nsp15 antibody [HL1446] (AB308423)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 (COVID 19) nsp15 using ab308423 at 1/1000 dilution (green). Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) nsp15 antibody [HL1446] (AB308423)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) nsp15 antibody [HL1446] (AB308423)

Immunohistochemical analysis of SARS-CoV-2 (COVID-19) nsp15 transfected HEK293T labeling SARS CoV 2 (COVID 19) nsp15 using ab308423 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-SARS CoV 2 (COVID 19) nsp15 antibody [HL1446] (AB308423)
  • WB

Supplier Data

Western blot - Anti-SARS CoV 2 (COVID 19) nsp15 antibody [HL1446] (AB308423)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp15 antibody [HL1446] (ab308423) diluted at 1/20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1446

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp15 also known as non-structural protein 15 is an endoribonuclease with uridine-specific activity. It is expressed in the cytoplasm of cells infected by the virus. Nsp15 has a monomer mass of approximately 42 kDa. In its active form it typically assembles into a hexameric complex which is important for its enzymatic function. It helps in evading the host immune response by degrading double-stranded RNA intermediates preventing detection by the host's antiviral pathways.
Biological function summary

Nsp15 is an important participant in the viral replication machinery of SARS-CoV-2. Nsp15 as part of the replication and transcription complex processes viral RNA to promote efficient replication. This protein may interact indirectly with other non-structural proteins such as nsp8 and nsp12 which form part of the RNA-dependent RNA polymerase complex. By maintaining the integrity of viral RNA nsp15 ensures successful virus propagation.

Pathways

Nsp15 plays a significant role in the viral life cycle particularly in the genome replication and transcription pathways. This protein is connected to nsp10 and nsp14 which are involved in RNA proofreading and capping. These interactions support the virus in overcoming innate immune responses by ensuring proper RNA processing and replication. Through these pathways nsp15 contributes significantly to the viral survival and proliferation within the host.

Nsp15 is primarily linked to COVID-19. Its role in immune evasion makes it a target of interest in the study of viral pathogenesis and therapeutic interventions. Research has indicated potential interactions between nsp15 and host proteins that could impact disease progression. Furthermore its manipulation or inhibition could serve as a therapeutic strategy potentially reducing the ability of SARS-CoV-2 to spread within the host. Understanding these interactions could aid in the development of antiviral drugs targeting nsp15 specifically.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 PLpro
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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