JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308357

Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SARS-CoV-2 (COVID-19) nsp7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301] (AB308357)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301] (AB308357)

Immunohistochemical analysis of SARS-CoV-2 (COVID-19) nsp7 transfected HEK293T cell FFPE Cell Pellet Block. labeling SARS CoV 2 COVID 19 nsp7 using ab308357 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301] (AB308357)
  • WB

Supplier Data

Western blot - Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301] (AB308357)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (ab308357) diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301] (AB308357)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp7 antibody [HL1301] (AB308357)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 nsp7 using ab308357 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1301

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, WB, IHC-P

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp7 also known as non-structural protein 7 is an essential component of the coronavirus replication machinery. This protein has a mass of approximately 83 amino acids and is expressed as part of the viral polyprotein after its synthesis in host cells. Nsp7 plays a mechanical role in stabilizing the SARS-CoV-2 replication complex where it acts alongside other non-structural proteins. It is not generated independently but is an integral element within infected host cells facilitating the viral replication process.
Biological function summary

The non-structural protein 7 of SARS-CoV-2 participates in forming a protein complex involving nsp8 and nsp12 to enhance viral RNA polymerase activity. This complex contributes to the processivity of the viral polymerase by extending the template RNA facilitating rapid and efficient viral RNA synthesis. Nsp7 through its interactions within this complex ensures the stability and proper functionality of the polymerase during viral replication. Its biological role is therefore indispensable in the production of viral progeny during infection.

Pathways

The involvement of nsp7 in viral RNA synthesis pathways is significantly linked to the replication and transcription mechanisms of SARS-CoV-2. Nsp7 in tandem with nsp8 modulates the activity of the RNA-dependent RNA polymerase (nsp12) important for the viral life cycle. This pathway directly impacts the replication fidelity and efficiency of SARS-CoV-2 ensuring successful propagation within the host. Through these interactions nsp7 not only stabilizes but also optimizes the function of the replication-transcription complex.

The activity of nsp7 is fundamental in the context of COVID-19 the disease caused by SARS-CoV-2 infection. The protein orchestrates parts of the viral replication process making it an indirect contributor to COVID-19 pathogenesis. Targeting the replication complex formed by nsp7 nsp8 and nsp12 can provide therapeutic strategies to combat COVID-19. The presence of nsp7 within the replication machinery implicates it in disease progression linking it to respiratory syndrome illnesses associated with SARS-CoV-2.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information SARS-CoV-2 (COVID-19) nsp7
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com