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AB308161

Anti-SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523]

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Rabbit Recombinant Monoclonal SARS-COV-2 nsp8 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate samples.
2 Images
Immunohistochemistry paraffin embedded sections - Anti-SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (AB308161)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (AB308161)

Immunohistochemical analysis of paraffin-embedded mock and SARS-CoV-2 Nsp8 transfected HEK-293T cell pellets labeling SARS-CoV-2 (COVID-19) nsp8 protein with ab308161 at 1/100 dilution (green). Cytoplasm staining on HEK-293T is observed. Heat mediated antigen retrieval with citrate buffer pH 6.0 for 15 mins. Nuclear DNA was labeled with Fluoroshield with DAPI (blue) Secondary antibody only control (left panels) : Primary diluent was used instead of primary antibody

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (AB308161)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (AB308161)

For immunofluorescence analysis, mock (left panels) and transfected (right panels) HEK-293T cells were fixed in 4% paraformaldehyde at room temperature for 15 min and permeabilized for detection of SARS-CoV-2 (COVID-19) nsp8 protein using ab308161 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1523

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

IHC-P, WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp8 also known as non-structural protein 8 forms part of the viral replicase complex of the severe acute respiratory syndrome coronavirus 2. Nsp8 has a mass of approximately 22 kDa and plays an important role in the RNA synthesis process of the virus. It is expressed inside host cells once the virus enters the host organism. The protein functions as a part of the RNA polymerase complex working alongside other non-structural proteins to enable the virus to replicate its genetic material effectively.
Biological function summary

Nsp8 cooperates with non-structural protein 12 (nsp12) to form the RNA-dependent RNA polymerase (RdRp) complex. This complex is essential for the replication and transcription of the viral genome. Nsp8 nsp12 and nsp7 come together as a multi-protein complex enhancing the activity of the RdRp. Nsp8 has been shown to stabilize the structure and function of the replication machinery providing the necessary support for the synthesis of the viral RNA strand.

Pathways

Nsp8 plays an important role in the viral replication and transcription pathways. The polymerase activity driven by the complex of nsp8 nsp12 and nsp7 operates through the replication-transcription complex important for efficient viral propagation within the host. This process not only involves the formation of viral RNA genomes but also subgenomic RNAs which are required for viral protein translation. The interaction between these non-structural proteins ensures that the virus maintains a high level of infectious presence in its host.

Nsp8 holds a strong connection to COVID-19 the disease caused by SARS-CoV-2 infection. The interplay between nsp8 and other non-structural proteins such as nsp12 contributes directly to the ability of the virus to replicate and cause widespread infection. Inhibition of nsp8 function could provide a therapeutic target for treating COVID-19 as disruption of the RdRp complex could hinder the viral replication process. Given the pivotal role of this protein in the virus’s lifecycle targeting nsp8 could offer promising avenues for antiviral drug development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-COV-2 nsp8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com