AB308365

Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399]

Recombinant
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Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

Immunohistochemical analysis of SARS-CoV-2 nsp9-transfected HEK293T labeling SARS CoV 2 COVID 19 nsp9 using ab308365 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 nsp9 using ab308365 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Supplier Data

Western blot - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] (ab308365) diluted at 1/10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (ab308365) at 1/10000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1399

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp9 also known as non-structural protein 9 plays an important role in viral replication. With a molecular weight of approximately 12 kDa this protein is integral to the replication and transcription complex of SARS-CoV-2 and is expressed during viral infection within host cells. Nsp9 facilitates the synthesis of RNA by binding to single-stranded RNA. This unique RNA-binding feature distinguishes it from other viral proteins implying its specific function in sustaining the viral genome.

Biological function summary

Nsp9 contributes significantly to the process of viral RNA replication. It functions within a complex that includes other non-structural proteins forming a multi-protein assembly necessary for efficient genome replication. This process ensures the viral RNA is correctly copied and translated therefore maintaining the infectivity of SARS-CoV-2. Nsp9's contribution to RNA binding and stabilization plays an important role in controlling the replication accuracy and speed of the virus.

Pathways

Nsp9 participates in the viral replication and transcription pathways of SARS-CoV-2. It interacts closely with proteins like nsp7 and nsp8 which are also a part of the replication machinery. These interactions within the RNA synthesis pathway facilitate the continuous production of viral components sustaining the life cycle of the virus within the host. Understanding these pathways provides insights into potential therapeutic targets to disrupt viral replication.

Nsp9 features prominently in COVID-19 the disease caused by SARS-CoV-2 infection. The protein's role in replication links it to the spread and severity of the infection. Targeting nsp9 could serve as a potential strategy for antiviral therapies. Moreover its interactions with other non-structural proteins such as nsp12 RNA-dependent RNA polymerase highlight the interconnectedness of these proteins in the progression of SARS-CoV-2-related pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SARS-CoV-2 PLpro

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

Western blot - Anti-SARS CoV 2 COVID 19 nsp9 antibody [HL1399] (AB308365)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise