Rabbit Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2, Recombinant fragment - SARS-CoV-2 samples.
Images
![Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--western-blot-img408276.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)")
![Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--western-blot-img408278.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)")
![Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--western-blot-img408277.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)")
![Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--immunocytochemistry-immunofluorescence-img408274.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Recombinant fragment - SARS-CoV-2 | Not recommended | Not recommended | Tested |
| SARS-CoV-2 | Predicted | Predicted | Predicted |
| Transfected cell line - SARS-CoV-2 | Tested | Tested | Not recommended |
| Transfected cell lysate - SARS-CoV-2 | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - SARS-CoV-2 | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - SARS-CoV-2, Recombinant fragment - SARS-CoV-2 | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - SARS-CoV-2 | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - SARS-CoV-2, Recombinant fragment - SARS-CoV-2 | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - SARS-CoV-2 | Dilution info 1/20000.00000 - 1/140000.00000 | Notes - |
Species Recombinant fragment - SARS-CoV-2 | Dilution info 1/20000.00000 - 1/140000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - SARS-CoV-2 | Dilution info - | Notes - |
Recommended products
Rabbit Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2, Recombinant fragment - SARS-CoV-2 samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1039
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SARS-CoV-2 Spike Glycoprotein S2 also known as S2 subunit is a segment of spike protein found on the surface of the SARS-CoV-2 virus. The spike protein facilitates viral entry into host cells. Mechanically S2 mediates membrane fusion which is essential for the virus to merge with cellular membranes allowing viral RNA to enter and replicate within the host. This protein has a molecular weight of approximately 680 kDa combined with S1. Expressed mainly in virions the spike protein is the target for many therapeutic interventions.
- Biological function summary
The SARS-CoV-2 Spike Glycoprotein S2 plays an important role in the viral infectivity process. It is part of a trimeric spike protein complex which undergoes a conformational shift during host cell entry. The S2 subunit specifically triggers the fusion process following receptor binding of another component the S1 subunit. During infection immune response typically generates anti-spike antibodies that target this protein aiming to block viral invasion or clear infected cells.
- Pathways
The SARS-CoV-2 Spike Glycoprotein S2 is critical in the viral entry and replication pathway. It interacts with host proteins in pathways involving endocytosis. The fusion activity of S2 is closely associated with cellular proteases like TMPRSS2 and furin which prime the spike protein for entry. These interactions are important for the initiation of the viral replication cycle and help shape the host's pathophysiological response to infection.
- Associated diseases and disorders
The SARS-CoV-2 Spike Glycoprotein S2 holds significant relevance in COVID-19 pathogenesis which is caused by the SARS-CoV-2 virus. This protein's interaction with angiotensin-converting enzyme 2 (ACE2) alongside the spike S1 subunit plays an important role in determining the virus’s infectivity and host cell invasion. Understanding its role can aid in designing therapeutic strategies against COVID-19 and related variants also revealing connections to host immune responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--western-blot-img408276.jpg "Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)")
Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)
All lanes: Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413) at 1/140000 dilution
All lanes: SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active)
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)
Immunohistochemical analysis of mock and SARS-CoV-2 (COVID-19) Spike transfected HEK293T cell FFPE Cell Pellet Block labeling SARS CoV 2 (COVID 19) Spike S2 / S2' using ab308413 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--western-blot-img408278.jpg "Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)")
Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1039] (ab308413) diluted at 1/20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413) at 1/20000 dilution
Lanes 1, 2 and 4: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 3: Transfected (–) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--western-blot-img408277.jpg "Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)")
Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody [HL1039] (ab308413) diluted at 1/100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413) at 1/100000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-covid-19-spike-s2-s2-antibody-hl1039-ab308413--immunocytochemistry-immunofluorescence-img408274.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)")
Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413)
For immunofluorescence analysis, mock and transfected SARS-CoV-2 (COVID-19) Spike transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 (COVID 19) Spike S2 / S2' using ab308413 at 1/1000 dilution (green).
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