AB308413

Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039]

Recombinant
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Rabbit Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2, Recombinant fragment - SARS-CoV-2 samples.

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Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

For immunofluorescence analysis, mock and transfected SARS-CoV-2 (COVID-19) Spike transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 (COVID 19) Spike S2 / S2' using ab308413 at 1/1000 dilution (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

Immunohistochemical analysis of mock and SARS-CoV-2 (COVID-19) Spike transfected HEK293T cell FFPE Cell Pellet Block labeling SARS CoV 2 (COVID 19) Spike S2 / S2' using ab308413 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody [HL1039] (ab308413) diluted at 1/100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413) at 1/100000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

All lanes:

Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (ab308413) at 1/140000 dilution

All lanes:

SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active)

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1039

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, IHC-P, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000 - 1/140000", "WB-species-notes": "" }, "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000 - 1/140000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 Spike Glycoprotein S2 also known as S2 subunit is a segment of spike protein found on the surface of the SARS-CoV-2 virus. The spike protein facilitates viral entry into host cells. Mechanically S2 mediates membrane fusion which is essential for the virus to merge with cellular membranes allowing viral RNA to enter and replicate within the host. This protein has a molecular weight of approximately 680 kDa combined with S1. Expressed mainly in virions the spike protein is the target for many therapeutic interventions.

Biological function summary

The SARS-CoV-2 Spike Glycoprotein S2 plays an important role in the viral infectivity process. It is part of a trimeric spike protein complex which undergoes a conformational shift during host cell entry. The S2 subunit specifically triggers the fusion process following receptor binding of another component the S1 subunit. During infection immune response typically generates anti-spike antibodies that target this protein aiming to block viral invasion or clear infected cells.

Pathways

The SARS-CoV-2 Spike Glycoprotein S2 is critical in the viral entry and replication pathway. It interacts with host proteins in pathways involving endocytosis. The fusion activity of S2 is closely associated with cellular proteases like TMPRSS2 and furin which prime the spike protein for entry. These interactions are important for the initiation of the viral replication cycle and help shape the host's pathophysiological response to infection.

The SARS-CoV-2 Spike Glycoprotein S2 holds significant relevance in COVID-19 pathogenesis which is caused by the SARS-CoV-2 virus. This protein's interaction with angiotensin-converting enzyme 2 (ACE2) alongside the spike S1 subunit plays an important role in determining the virus’s infectivity and host cell invasion. Understanding its role can aid in designing therapeutic strategies against COVID-19 and related variants also revealing connections to host immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SARS-CoV-2 Spike Glycoprotein S2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039]

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

Western blot - Anti-SARS CoV 2 (COVID 19) Spike S2 / S2' antibody [HL1039] (AB308413)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise