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AB313850

Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free

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Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free (AB313850)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free (AB313850)

Immunofluorescent analysis of mock and SARS-CoV-2 (COVID-19) nsp15 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling SARS-CoV-2 (COVID-19) nsp15 with ab313850 at 1/1000 dilution (green). Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free (AB313850)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free (AB313850)

Immunohistochemical analysis of paraffin-embedded SARS-CoV-2 (COVID-19) nsp15 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling SARS-CoV-2 (COVID-19) nsp15 with ab313850 at 1/1000 dilution (green). Blue : Fluoroshield with DAPI. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.

Western blot - Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free (AB313850)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free (AB313850)

All lanes:

Western blot - Anti-SARS-CoV-2 nsp15 antibody [HL1445] - BSA and Azide free (ab313850) at 1/1000 dilution

Lane 1:

Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

DDDDK-tag tagged SARS-CoV-2 (COVID19) nsp15 transfected HEK293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1445

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

IHC-P, ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp15 also known as non-structural protein 15 is an endoribonuclease with uridine-specific activity. It is expressed in the cytoplasm of cells infected by the virus. Nsp15 has a monomer mass of approximately 42 kDa. In its active form it typically assembles into a hexameric complex which is important for its enzymatic function. It helps in evading the host immune response by degrading double-stranded RNA intermediates preventing detection by the host's antiviral pathways.
Biological function summary

Nsp15 is an important participant in the viral replication machinery of SARS-CoV-2. Nsp15 as part of the replication and transcription complex processes viral RNA to promote efficient replication. This protein may interact indirectly with other non-structural proteins such as nsp8 and nsp12 which form part of the RNA-dependent RNA polymerase complex. By maintaining the integrity of viral RNA nsp15 ensures successful virus propagation.

Pathways

Nsp15 plays a significant role in the viral life cycle particularly in the genome replication and transcription pathways. This protein is connected to nsp10 and nsp14 which are involved in RNA proofreading and capping. These interactions support the virus in overcoming innate immune responses by ensuring proper RNA processing and replication. Through these pathways nsp15 contributes significantly to the viral survival and proliferation within the host.

Nsp15 is primarily linked to COVID-19. Its role in immune evasion makes it a target of interest in the study of viral pathogenesis and therapeutic interventions. Research has indicated potential interactions between nsp15 and host proteins that could impact disease progression. Furthermore its manipulation or inhibition could serve as a therapeutic strategy potentially reducing the ability of SARS-CoV-2 to spread within the host. Understanding these interactions could aid in the development of antiviral drugs targeting nsp15 specifically.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 nsp15
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com