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AB272852

Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric)

5

(3 Reviews)

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(8 Publications)

Human Recombinant Monoclonal NCAP antibody. Suitable for ELISA, WB and reacts with SARS-CoV-2 samples. Cited in 8 publications.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

2 Images
ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (AB272852)
  • ELISA

Supplier Data

ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (AB272852)

Binding Activity of ab272852 with SARS-CoV-2 nucleocapsid protein.

Activity : Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 nucleocapsid protein at 5 μg/ml can bind ab272852. The EC50 of ab272852 is 43.50-118.4 ng/ml.

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (AB272852)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (AB272852)

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) at 1/1000 dilution

All lanes:

His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli, 200 ng

Secondary

All lanes:

Peroxidase Goat Anti-Human IgG, Fcγ Fragment Specific at 1/20000 dilution

false

  • HRP

    HRP Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric)

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

1A6

Isotype

IgG1

Carrier free

No

Reacts with

SARS-CoV-2

Applications

WB, ELISA

applications

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Reactivity data

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Product details

Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.

As the Fc region is human, an anti-Human secondary antibody should be used for detection.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
See full target information N
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular biology 3:e70014 PubMed39619685

2024

Comparison of localization and release of multivesicular bodies and secretory granules in islet cells: Dysregulation during type-2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Priyadarshini Veerabhadraswamy,Kiran Lata,Sristi Dey,Prajakta Belekar,Lakshmi Kothegala,Vidya Mangala Prasad,Nikhil R Gandasi

International journal of molecular sciences 23: PubMed36142365

2022

Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Thatiana Corrêa de Melo,Dilza Trevisan-Silva,Miryam P Alvarez-Flores,Renata Nascimento Gomes,Marcelo Medina de Souza,Hellen Paula Valerio,Douglas S Oliveira,Carlos DeOcesano-Pereira,Viviane Fongaro Botosso,Soraia Attie Calil Jorge,Mirta Schattner,Ricardo M Gomez,Ana Marisa Chudzinski-Tavassi

Cell 185:881-895.e20 PubMed35216672

2022

Multiple early factors anticipate post-acute COVID-19 sequelae.

Applications

Unspecified application

Species

Unspecified reactive species

Yapeng Su,Dan Yuan,Daniel G Chen,Rachel H Ng,Kai Wang,Jongchan Choi,Sarah Li,Sunga Hong,Rongyu Zhang,Jingyi Xie,Sergey A Kornilov,Kelsey Scherler,Ana Jimena Pavlovitch-Bedzyk,Shen Dong,Christopher Lausted,Inyoul Lee,Shannon Fallen,Chengzhen L Dai,Priyanka Baloni,Brett Smith,Venkata R Duvvuri,Kristin G Anderson,Jing Li,Fan Yang,Caroline J Duncombe,Denise J McCulloch,Clifford Rostomily,Pamela Troisch,Jing Zhou,Sean Mackay,Quinn DeGottardi,Damon H May,Ruth Taniguchi,Rachel M Gittelman,Mark Klinger,Thomas M Snyder,Ryan Roper,Gladys Wojciechowska,Kim Murray,Rick Edmark,Simon Evans,Lesley Jones,Yong Zhou,Lee Rowen,Rachel Liu,William Chour,Heather A Algren,William R Berrington,Julie A Wallick,Rebecca A Cochran,Mary E Micikas,Terri Wrin,Christos J Petropoulos,Hunter R Cole,Trevan D Fischer,Wei Wei,Dave S B Hoon,Nathan D Price,Naeha Subramanian,Joshua A Hill,Jennifer Hadlock,Andrew T Magis,Antoni Ribas,Lewis L Lanier,Scott D Boyd,Jeffrey A Bluestone,Helen Chu,Leroy Hood,Raphael Gottardo,Philip D Greenberg,Mark M Davis,Jason D Goldman,James R Heath

International journal of biological macromolecules 200:428-437 PubMed35051498

2022

Dimerization of SARS-CoV-2 nucleocapsid protein affects sensitivity of ELISA based diagnostics of COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Wajihul Hasan Khan,Nida Khan,Avinash Mishra,Surbhi Gupta,Vikrant Bansode,Deepa Mehta,Rahul Bhambure,M Ahmad Ansari,Shukla Das,Anurag S Rathore

Nature communications 12:6483 PubMed34759292

2021

A Fabry-Pérot cavity coupled surface plasmon photodiode for electrical biomolecular sensing.

Applications

Unspecified application

Species

Unspecified reactive species

Giles Allison,Amrita Kumar Sana,Yuta Ogawa,Hidemi Kato,Kosei Ueno,Hiroaki Misawa,Koki Hayashi,Hironori Suzuki

JACS Au 1:1147-1157 PubMed34462738

2021

Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Corinne A Lutomski,Tarick J El-Baba,Jani R Bolla,Carol V Robinson

EBioMedicine 67:103348 PubMed33906067

2021

A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Klausberger,Mark Duerkop,Helmuth Haslacher,Gordana Wozniak-Knopp,Monika Cserjan-Puschmann,Thomas Perkmann,Nico Lingg,Patricia Pereira Aguilar,Elisabeth Laurent,Jelle De Vos,Manuela Hofner,Barbara Holzer,Maria Stadler,Gabriele Manhart,Klemens Vierlinger,Margot Egger,Lisa Milchram,Elisabeth Gludovacz,Nicolas Marx,Christoph Köppl,Christopher Tauer,Jürgen Beck,Daniel Maresch,Clemens Grünwald-Gruber,Florian Strobl,Peter Satzer,Gerhard Stadlmayr,Ulrike Vavra,Jasmin Huber,Markus Wahrmann,Farsad Eskandary,Marie-Kathrin Breyer,Daniela Sieghart,Peter Quehenberger,Gerda Leitner,Robert Strassl,Alexander E Egger,Christian Irsara,Andrea Griesmacher,Gregor Hoermann,Günter Weiss,Rosa Bellmann-Weiler,Judith Loeffler-Ragg,Nicole Borth,Richard Strasser,Alois Jungbauer,Rainer Hahn,Jürgen Mairhofer,Boris Hartmann,Nikolaus B Binder,Gerald Striedner,Lukas Mach,Andreas Weinhäusel,Benjamin Dieplinger,Florian Grebien,Wilhelm Gerner,Christoph J Binder,Reingard Grabherr

Nature microbiology 6:339-353 PubMed33349665

2020

The SARS-CoV-2 RNA-protein interactome in infected human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nora Schmidt,Caleb A Lareau,Hasmik Keshishian,Sabina Ganskih,Cornelius Schneider,Thomas Hennig,Randy Melanson,Simone Werner,Yuanjie Wei,Matthias Zimmer,Jens Ade,Luisa Kirschner,Sebastian Zielinski,Lars Dölken,Eric S Lander,Neva Caliskan,Utz Fischer,Jörg Vogel,Steven A Carr,Jochen Bodem,Mathias Munschauer
View all publications
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