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Human Recombinant Monoclonal NCAP antibody. Suitable for ELISA, WB and reacts with SARS-CoV-2 samples. Cited in 6 publications.


Images

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (AB272852), expandable thumbnail
  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (AB272852), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Human

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
SARS-CoV-2
Tested
Tested

Tested
Tested

Species

SARS-CoV-2

Dilution info

-

Notes

-

Tested
Tested

Species

SARS-CoV-2

Dilution info

1/500 - 1/5000

Notes

-

Target data

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661).May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414).May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

Alternative names

Recommended products

Human Recombinant Monoclonal NCAP antibody. Suitable for ELISA, WB and reacts with SARS-CoV-2 samples. Cited in 6 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

1A6

Purification technique

Affinity purification

Concentration
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Purification notes

Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.

As the Fc region is human, an anti-Human secondary antibody should be used for detection.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

Associated diseases and disorders

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852), expandable thumbnail

    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)

    All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852) at 1/1000 dilution

    All lanes: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli, 200 ng

    Secondary

    All lanes: Peroxidase Goat Anti-Human IgG, Fcγ Fragment Specific at 1/20000 dilution

  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852), expandable thumbnail

    ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Chimeric (ab272852)

    Binding Activity of ab272852 with SARS-CoV-2 nucleocapsid protein.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 nucleocapsid protein at 5 μg/ml can bind ab272852. The EC50 of ab272852 is 43.50-118.4 ng/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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