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Rabbit Recombinant Monoclonal NCAP antibody. Suitable for WB, ELISA, sELISA and reacts with Recombinant fragment - SARS-CoV-2, Recombinant full length protein - SARS-CoV-2 samples.

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Images

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284653), expandable thumbnail
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284653), expandable thumbnail
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284653), expandable thumbnail
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284653), expandable thumbnail
  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284653), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 98% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBELISAsELISA
Recombinant fragment - SARS-CoV-2
Tested
Not recommended
Not recommended
Recombinant full length protein - SARS-CoV-2
Not recommended
Tested
Tested
SARS-CoV-2
Predicted
Predicted
Predicted

Tested
Tested

Species
Recombinant fragment - SARS-CoV-2
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
SARS-CoV-2
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - SARS-CoV-2
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - SARS-CoV-2
Dilution info
0.00014-1.00000 µg/mL
Notes

-

Predicted
Predicted

Species
SARS-CoV-2
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - SARS-CoV-2
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - SARS-CoV-2
Dilution info
0.00014-1.00000 µg/mL
Notes

-

Predicted
Predicted

Species
SARS-CoV-2
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - SARS-CoV-2
Dilution info
-
Notes

-

Target data

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661). May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414). May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

Alternative names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

Recommended products

Rabbit Recombinant Monoclonal NCAP antibody. Suitable for WB, ELISA, sELISA and reacts with Recombinant fragment - SARS-CoV-2, Recombinant full length protein - SARS-CoV-2 samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Clone number
75G5a
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

Associated diseases and disorders

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653), expandable thumbnail

    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653)

    All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653) at 1 µg/mL

    Lane 1: SARS-CoV-2 nucleocapsid protein, 50 ng

    Lane 2: SARS-CoV nucleocapsid protein, 50 ng

  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653), expandable thumbnail

    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653)

    Multiple sandwich ELISA's were performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: ab284653 (75G5a/ blue) as a capture antibody and various detection antibodies for COVID-19 84C4a (orange) or 84D7 (grey) or SARS-CoV clones 75G5a (yellow), 84C4a (blue), 84D7 (green). COVID-19 NP was serially diluted 1/2 starting at 25 ng/mL. Rabbit monoclonal antibodies for COVID-19 clones, including ab284653 (75G5a), detected COVID-19 NP antigen at very high sensitivity as low as 100 pg/mL (10 pg). SARS-CoV clones did not detect the COVID-19 NP antigen.

  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653), expandable thumbnail

    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653)

    A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: ab284653 (75G5a) as a capture antibody and Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [85C10] ab284656 (85C1-biotin) as a detection antibody. COVID-19 NP was serially diluted 1/2 starting at 25 ng/mL. Rabbit monoclonal antibodies ab284653 (75G5a) and 85C1-biotin detected COVID-19 NP antigen at very high sensitivity as low as 100 pg/mL (10 pg).

  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653), expandable thumbnail

    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653)

    A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: ab284653 (75G5a) as a capture antibody and Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [85C10] ab284656 (85C1-biotin) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies ab284653 (75G5a) and Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [85C10] ab284656 (85C1-biotin) detected COVID-19 NP antigen at very high sensitivity as low as 100 pg/mL (10 pg).

  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653), expandable thumbnail

    ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653)

    Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 μg/mL.

    ab284653 was serially diluted 1/2 starting at 1 μg/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.

  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653), expandable thumbnail

    ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (ab284653)

    Affinity measurement of ab284653 (SARS-CoV-2 (75Ga) antibody).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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