Rabbit Polyclonal NCAP antibody. Suitable for WB and reacts with SARS-CoV-2 samples. Cited in 4 publications.
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Publications
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- International journal of molecular sciences 24:2023Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlo Brogna,Vincenzo Costanzo,Barbara Brogna,Domenico Rocco Bisaccia,Giancarlo Brogna,Marino Giuliano,Luigi Montano,Valentina Viduto,Simone Cristoni,Mark Fabrowski,Marina PiscopoPubMed 36835341
- Canadian Urological Association journal = Journal de l'Association des urologues du Canada 16:E558-E5622022Case series - COVID-19 is unlikely to affect male fertility: Results of histopathological and reverse transcriptase polymerase chain reaction analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn M Masterson,Chau Bui,Wohaib Hasan,Yi Zhang,Carissa Huynh,Harneet Jawanda,Daniel Luthringer,Warren Tourtellotte,Eric Vail,Maurice M GarciaPubMed 35704934
- Vaccines 10:2022Could SARS-CoV-2 Have Bacteriophage Behavior or Induce the Activity of Other Bacteriophages?Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlo Brogna,Barbara Brogna,Domenico Rocco Bisaccia,Francesco Lauritano,Giuliano Marino,Luigi Montano,Simone Cristoni,Marina Prisco,Marina PiscopoPubMed 35632464
- Veterinary pathology 59:602-6122022Comparative pathology of the nasal epithelium in K18-hACE2 Tg mice, hACE2 Tg mice, and hamsters infected with SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPin Yu,Wei Deng,Linlin Bao,Yajin Qu,Yanfeng Xu,Wenjie Zhao,Yunlin Han,Chuan QinPubMed 35094625
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Tris HCl- Form
- Liquid
- Clonality
- Polyclonal
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Reactivity data
Select an application| WB | |
|---|---|
| SARS-CoV-2 | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Target data
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661). May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414). May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
Alternative names
Nucleoprotein, N, Nucleocapsid protein, NC, Protein N
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Rabbit Polyclonal NCAP antibody. Suitable for WB and reacts with SARS-CoV-2 samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Tris HCl- Form
- Liquid
- Clonality
- Polyclonal
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is manufactured by BioVision, an Abcam company and was previously called A2061 Anti-SARS-CoV-2 Antibody. A2061-50 is the same size as the 50 μg size of ab273167.
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.
- Biological function summary
The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.
- Pathways
The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.
- Associated diseases and disorders
The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody (ab273167)
All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody (ab273167) at 2 µg/mL
All lanes: Purified recombinant Nucleoprotein from SARS-CoV-2
Predicted band size: 50 kDa
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