AB273167

Anti-SARS-CoV-2 nucleocapsid protein antibody

(2 Reviews)

(13 Publications)

Rabbit Polyclonal NCAP antibody. Suitable for WB and reacts with SARS-CoV-2 samples. Cited in 13 publications.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

1 Images

Supplier Data

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody (AB273167)

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody (ab273167) at 2 µg/mL

All lanes:

Purified recombinant Nucleoprotein from SARS-CoV-2

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

SARS-CoV-2

Applications

applications

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Reactivity data

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called A2061 Anti-SARS-CoV-2 Antibody. A2061-50 is the same size as the 50 μg size of ab273167.

Applications overview

Tick: Tested and Guaranteed to work X: Will not work —: No data

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Properties and storage information

Form

Liquid

Purity

undefined SDS-PAGE

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 0.87% Sodium chloride, 0.16% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation (PubMed : 39225180).

See full target information N

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

F1000Research 11:292 PubMed40444030

2025

Evidence of SARS-CoV-2 bacteriophage potential in human gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Mauro Petrillo,Maddalena Querci,Carlo Brogna,Jessica Ponti,Simone Cristoni,Peter V Markov,Andrea Valsesia,Gabriele Leoni,Alessandro Benedetti,Thierry Wiss,Guy Van den Eede

Microorganisms 12: PubMed38674588

2024

Who Is the Intermediate Host of RNA Viruses? A Study Focusing on SARS-CoV-2 and Poliovirus.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Brogna,Domenico Rocco Bisaccia,Vincenzo Costanzo,Gennaro Lettieri,Luigi Montano,Valentina Viduto,Mark Fabrowski,Simone Cristoni,Marina Prisco,Marina Piscopo

Frontiers in drug delivery 4:1359700 PubMed40836990

2024

Single cell phototransfection of mRNAs encoding SARS-CoV2 spike and nucleocapsid into human astrocytes results in RNA dependent translation interference.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Bum Kim,Quentin Brosseau,Julia Radzio,Jinhui Wang,Hiromi Muramatsu,Da Kuang,M Sean Grady,H Isaac Chen,John A Wolf,Alexandra V Ulyanova,Tamas Bartfai,Junhyong Kim,Norbert Pardi,Jai-Yoon Sul,Paulo Arratia,James Eberwine

Viruses 15: PubMed37515096

2023

LSDV-Vectored SARS-CoV-2 S and N Vaccine Protects against Severe Clinical Disease in Hamsters.

Applications

Unspecified application

Species

Unspecified reactive species

Warren R J de Moor,Anna-Lise Williamson,Georgia Schäfer,Nicola Douglass,Sophette Gers,Andrew D Sutherland,Melissa J Blumenthal,Emmanuel Margolin,Megan L Shaw,Wolfgang Preiser,Rosamund Chapman

International journal of molecular sciences 24: PubMed36835341

2023

Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Brogna,Vincenzo Costanzo,Barbara Brogna,Domenico Rocco Bisaccia,Giancarlo Brogna,Marino Giuliano,Luigi Montano,Valentina Viduto,Simone Cristoni,Mark Fabrowski,Marina Piscopo

Molecular therapy : the journal of the American Society of Gene Therapy 31:344-361 PubMed36514292

2022

Treatment with quercetin inhibits SARS-CoV-2 N protein-induced acute kidney injury by blocking Smad3-dependent G1 cell-cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Wu,Wenbiao Wang,Liying Liang,Junzhe Chen,Biao Wei,Xiao-Ru Huang,Xiaoqin Wang,Xueqing Yu,Hui-Yao Lan

Diagnostics (Basel, Switzerland) 12: PubMed36359457

2022

Functionalized Titanium Dioxide Nanoparticle-Based Electrochemical Immunosensor for Detection of SARS-CoV-2 Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Mohd Abubakar Sadique,Shalu Yadav,Vedika Khare,Raju Khan,Gagan Kant Tripathi,Purnima Swarup Khare

Canadian Urological Association journal = Journal de l'Association des urologues du Canada 16:E558-E562 PubMed35704934

2022

Case series - COVID-19 is unlikely to affect male fertility: Results of histopathological and reverse transcriptase polymerase chain reaction analysis.

Applications

Unspecified application

Species

Unspecified reactive species

John M Masterson,Chau Bui,Wohaib Hasan,Yi Zhang,Carissa Huynh,Harneet Jawanda,Daniel Luthringer,Warren Tourtellotte,Eric Vail,Maurice M Garcia

ACS applied bio materials 5:2421-2430 PubMed35522141

2022

Highly Sensitive Electrochemical Immunosensor Platforms for Dual Detection of SARS-CoV-2 Antigen and Antibody based on Gold Nanoparticle Functionalized Graphene Oxide Nanocomposites.

Applications

Unspecified application

Species

Unspecified reactive species

Mohd Abubakar Sadique,Shalu Yadav,Pushpesh Ranjan,Raju Khan,Firoz Khan,Ashok Kumar,Debasis Biswas

Vaccines 10: PubMed35632464

2022

Could SARS-CoV-2 Have Bacteriophage Behavior or Induce the Activity of Other Bacteriophages?

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Brogna,Barbara Brogna,Domenico Rocco Bisaccia,Francesco Lauritano,Giuliano Marino,Luigi Montano,Simone Cristoni,Marina Prisco,Marina Piscopo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS-CoV-2 nucleocapsid protein antibody

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody (AB273167)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise