Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ELISA, WB and reacts with SARS-CoV-2 samples.
Images
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (AB288321), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-abk79-e11-bsa-and-azide-free-ab288321--western-blot-img176620.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (AB288321)")
![ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (AB288321), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-abk79-e11-bsa-and-azide-free-ab288321--elisa-img170433.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (AB288321)")
Key facts
- Isotype
- IgG1
- Host species
- Human
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| SARS-CoV-2 | Tested | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info 0-0.1 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info - | Notes - |
Target data
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661). May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414). May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
Alternative names
Nucleoprotein, N, Nucleocapsid protein, NC, Protein N
Recommended products
Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ELISA, WB and reacts with SARS-CoV-2 samples.
Key facts
- Isotype
- IgG1
- Host species
- Human
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- ABK79-E11
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab288321 is the carrier free version of Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.
- Biological function summary
The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.
- Pathways
The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.
- Associated diseases and disorders
The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (ab288321), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-abk79-e11-bsa-and-azide-free-ab288321--western-blot-img176620.jpg "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (ab288321)")
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (ab288321)
This data was developed using the same antibody clone in a different buffer formulation (Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894).
Lanes 1 - 2: Green - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894 observed at 51 kDa. Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894 was shown to react with SARS-CoV-2 N-protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Human 800CW secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] (Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894)
Lane 1: Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530) at 0.2 µg
Lane 2: Recombinant Human coronavirus SARS Nucleocapsid Protein at 0.2 µg
SecondaryAll lanes: Goat anti-Human 800CW at 1/20000 dilution
![ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (ab288321), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-abk79-e11-bsa-and-azide-free-ab288321--elisa-img170433.jpg "ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (ab288321)")
ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - BSA and Azide free (ab288321)
This data was developed using the same antibody clone in a different buffer formulation (Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894)
Human monoclonal [ABK79-E11] to SARS-CoV-2 nucleocapsid protein (Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894)
Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530) at 1000 ng/ml.
Primary antibody (Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] ab283894) concentration range 0.045-100 ng/mL.
Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (Goat Anti-Human IgG Fc (HRP) preadsorbed ab98624) used at 1/1000 dilution.
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