Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric)

• ELISA

Lab

ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric) (AB283895)

Mouse monoclonal [ABK79-E11] to SARS-CoV-2 nucleocapsid protein – Chimeric (ab283895)
Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) at 1000 ng/ml.
Primary antibody (ab283894) concentration range 0.045-100 ng/mL.
Secondary antibody, Goat anti-Mouse IgG Fc (HRP) used at 1/2000 dilution.

• WB

Lab

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric) (AB283895)

False colour image of Western blot : Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] – Mouse IgG2a (Chimeric) staining at 1/1000 dilution, shown in green. In Western blot, ab283895 was shown to bind specifically to SARS-CoV-2 nucleocapsid protein. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) at 1/20000 dilution.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric) (ab283895)

All lanes:

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) <a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a> cell lysate at 0.2 µg

Observed band size: 51 kDa

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• WB

Lab

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric) (AB283895)

Lanes 1 - 2 : Green - ab283895 observed at 51 kDa.ab283895 was shown to react with SARS-CoV-2 N-protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab283894 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Mouse 800CW secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric) (ab283895) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Lane 2:

Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.2 µg

Secondary

All lanes:

Goat anti-Mouse 800CW at 1/20000 dilution

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