AB288323

Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free

Recombinant
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Mouse Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ELISA, WB and reacts with SARS-CoV-2 samples.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

3 Images

ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free (AB288323)

ELISA

Lab

ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free (AB288323)

This data was developed using the same antibody clone in a different buffer formulation (ab283895)

Mouse monoclonal [ABK79-E11] to SARS-CoV-2 nucleocapsid protein – Chimeric (ab283895)
Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) at 1000 ng/ml.
Primary antibody (ab283894) concentration range 0.045-100 ng/mL.
Secondary antibody, Goat anti-Mouse IgG Fc (HRP) used at 1/2000 dilution.

Lab

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free (AB288323)

This data was developed using the same antibody clone in a different buffer formulation (ab283895)

False colour image of Western blot : Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] – Mouse IgG2a (Chimeric) staining at 1/1000 dilution, shown in green. In Western blot, ab283895 was shown to bind specifically to SARS-CoV-2 nucleocapsid protein. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) at 1/20000 dilution.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-nucleocapsid-protein-antibody-abk79-e11-mouse-igg2a-chimeric-ab283895'>ab283895</a>)

All lanes:

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) <a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a> cell lysate at 0.2 µg

Observed band size: 51 kDa

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Lab

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free (AB288323)

This data was developed using the same antibody clone in a different buffer formulation (ab283895)

Lanes 1 - 2 : Green - ab283895 observed at 51 kDa.ab283895 was shown to react with SARS-CoV-2 N-protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab283894 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Mouse 800CW secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Lane 2:

Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.2 µg

Secondary

All lanes:

Goat anti-Mouse 800CW at 1/20000 dilution

false

Unconjugated

Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ABK79-E11

Isotype

IgG2a

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

This mouse monoclonal chimeric antibody (ab288323) is the carrier free version of ab283895.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

See full target information N

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free

ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free (AB288323)

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free (AB288323)

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric), BSA & Azide free (AB288323)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise